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Hello, I don't understand why there is this error message on Amos... My data are from a survey and on SPSS everything is fine... Could you help me?

21 February 2020 3,001 3 View

Are there any studies that explore the relationship between cuticle thickness and body size in species of decapod crustaceans?

This relationship is very obvious to me (think of lobsters), but hard to actually measure. I could not find any study which addresses this issue!

11 September 2017 1,995 14 View

Heat mediated antigen retrieval (using a microwave) for Olig2 immunohistochemistry staining?

11 January 2017 4,314 6 View

We are making a frozen stock, how much 20% glycerol should we add to 5ml spinned down overnight culture?

We need to grow 5ml of overnight culture according to the manufacturer. Then the protocol we have says to spin down the bacterial cells, but it doesn't mention the volume for reconstitution, is it...

30 November 2015 376 3 View

Do you know of any technique to separate chlorophylls from the plant extract?

The extracts are ethanolic and methanolic. I tried a partition with petroleum ether and hexane with very poor results. I need any faster technique than the column chromatography, The extracts are...

11 November 2015 9,531 3 View

What are the countries most affected by extreme rainfall as a result of climate change?

Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...

01 March 2021 7,168 9 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View

Are these moths Spodoptera frugiperda?

The caterpillars of the moths were collected as fruitborers in pepper at a farmer. I reared them till the adults hatched and compared them with pictures and descriptions on the internet. Are these...

24 February 2021 7,785 5 View

Please help in identification of this insect?

This insect is minute in size around 1 mm and not clearly visible with naked eye.

22 February 2021 5,907 11 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View

Extraction of DNA from insect samples conserved in silica gel?

Hi everybody, can anybody suggest how to extract DNA from insect samples conserved in silica gel (in my case, mosquitoes)? Does anybody know a commericial kit which does that or a particular...

10 February 2021 3,392 4 View

How do I prepare and store insect samples for JH measurements?

I need to preserve termite samples for future quantification of juvenile hormone in their bodies. Or extract the Juvenile Hormone and keep that samples stored for future quantification. I may need...

09 February 2021 1,995 1 View

Working with insect primary cells in cell medium and in saline solution?

I work with insect primary cells, which I let grow in TC100 insect medium. For fluorescence measurements I have to discard medium (because of its fluorescence properties) and give a saline...

28 January 2021 3,867 1 View

How to extract a single stranded DNA from a double stranded DNA sample isolated from stem borer(rice affecting insect) ?

How to extract a single-stranded DNA from a double-stranded DNA sample isolated from stem borer(rice affecting insect)? As I need to amplify ss Dna using a COX-1 marker having primer sequence as...

22 January 2021 3,424 3 View

Helicoverpa armigera eggs are not hatching in lab condition, why?

Dear RG friends, I am providing a complete H. armigera diet for larvae and maintaining the eggs in the growth chamber as well as in-room conditions. But since three months, eggs laid from the...

21 January 2021 5,546 32 View