How to make graphene oxide film on substrate for electrochemical performance?

I am trying to deposit graphene oxide film on steel electrode via drop casting (Electrochemical synthesis of layer-by-layer reduced graphene... ) and dry it in oven at 60 C. when i put this into...

04 December 2020 820 2 View

If anyone can suggest or review manuscript having keywords Riparian zone, River, Species richness, Species evenness, Ornithofauna?

Greetings I am Sachin Kumar, pursuing my Ph.D. in Ornithology, from PAU, Ludhiana. I am working on avian diversity along the River Beas. I had submitted a manuscript to a journal having keywords...

10 November 2020 7,194 6 View

Can anyone provide me .pdb file for Spike protein of SARS Cov-2, ACE2 complex and TMPRSS2?

Dear Research Colleagues, I am looking for the .pdb file for Spike protein of SARS Cov-2, ACE2 complex and TMPRSS2. I have to use them for Molecular Docking analysis of synthetic organic compounds...

08 September 2020 8,788 3 View

Can i use Ag/AgCl reference electrode to electrodeposite silver?

I'm trying to electrodeposit silver nanoparticle from aqueous solution of AgNO3 via three electrode configuration. When i dip three electrode in aqueous AgNO3 solution, a whitish fluid coming out...

22 August 2020 6,837 7 View

Can i use Graphite flakes having 325 mesh particle size instead of Carbon Black or acetylene to make slurry for electrochemical performance ?

Can i use Graphite flakes having 325 mesh particle size as conductive agent instead of acetylene black or carbon black to make slurry for super capacitor electrode ?

27 July 2020 1,176 1 View

Why bubbles appear on film during Galvaostatic charging discharging?

I electrodeposited GO/PANI directly on electrode and dried at room temperature, then used it for Galvanostatic charge discharge via three electrode configuration in 1 M H2SO4. During charging...

20 July 2020 5,988 8 View

Is it necessary to do Open circuit potential before CV, Galvanostatic charge discharge and EIS for Supercapacitor electrode?

for electrochemical performance of supercapacitor electrode i am doing CV, Galvanostatic charge discharge and Potentiostatic EIS via three electrode configuration. is it necessary to do open...

19 June 2020 2,026 7 View

Does metal to metal joining by FSW really occur so as to withstand loads in abaqus?

I am curious to know if metal bonding by welding (FSW) can really be simulated in Abaqus or not. I have read many papers wherein people simulate the FSW process only and study the temperature and...

15 June 2020 6,471 1 View

Which is correct formula to calculate gravimetric specific capacitance from Galvanostatic charge discharge method?

I have to calculate specific capacitance (F/g) from galvanostatic charge discharge. in electrochemical test, one is working electrode, platinum sheet as counter electrode and Ag/AgCl as reference...

07 June 2020 5,213 3 View

How to develop 3D setup for abrasive jet machining in ansys fluent ?

hello everyone....... I am trying to develop 3D setup of abrasive jet machining (AJM) which consist of nozzle from which stream of air+abrasive (two phase flow) strike the workpiece and erosion...

30 May 2020 2,136 8 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

I can detect my protein but the encoding DNA sequence has stop codons within. Should I ignore the stop codons?

I constructed a synthetic DNA library (scFv, VH-VL orientation) with a 3' reporter and 8x histag. I cloned and expressed this gene following which I performed an ELISA. The ELISA results suggested...

19 February 2021 7,006 5 View

Purification of proteins from baculovirus-infected insect cells?

18 February 2021 6,665 6 View