Sachin Teotia

10 Questions 17 Answers 0 Followers

Questions related from Sachin Teotia

How to germinate 3-4 years old rice (Japonica) seeds stored at room temperature?

There are problems germinating old transgenic rice seeds (Japonica). Some seeds are 4 years old, while others are 3. They were stored at room temperature. Can we treat them in a way to have at...

15 June 2019 5,821 3 View

What is the safe fold change to consider in a RNA-seq experiment?

What is the safe fold change to consider in a RNA-seq experiment? Fold change > 1.5, FDR < 0.05, P-value < 0.05 and 'Test status' = OK is one criteria which was taken, but I have also...

04 November 2015 3,060 12 View

Is it possible to silence a transgene (driven by a strong promoter) by an artificial miRNA driven by an inducible promoter in Arabidopsis?

While in literature there is no report, to my knowledge, to silence one transgene in Arabidopsis (under 35S promoter) using another transgene (artificial microRNA, under inducible system). I think...

27 June 2015 9,456 1 View

How can I generate a heatmap and clustering of differentially expressed genes in a RNA-seq data?

I have RNA-seq data of rice with some 12000 differentially expressed genes. How to make heat maps and clusters from that data?

26 June 2015 4,641 30 View

What is the difference between strand-specific and not strand-specific RNA-seq data?

What is the difference between strand-specific and not strand-specific RNA-seq data? Is non-strand specific the regular polyA-based mRNA library? And how these two seq-libraries differ...

15 April 2015 7,374 11 View

How to select Arabidopsis seeds using BASTA on MS medium?

I want to select transgenic Arabidopsis seeds using BASTA on MS medium. My mutant does not survive well on the soil and want to initially select the T0 seeds on BASTA in MS medium. Can anyone tell...

30 March 2015 9,103 7 View

Is it necessary to treat plant RNA with DNase for RNA-seq experiment?

I am preparing RNA from plant samples for RNA-seq. Is it necessary to treat the RNA with DNAse (which will be done once RNA is isolated)? My concern is, extra step of DNase treatment and then...

22 March 2015 1,128 7 View

While doing VIGS, can the infiltrated virus spread from the leaves to the germline tissues and knockdown the germline gene?

We have seen VIGS induced silencing of PDS, GFP and many other genes in many species. Is there any report or is it possible that we can knockdown a gene expressed in germline tissues using VIGS?...

14 January 2015 3,615 3 View

How do you get rid of condensation from petriplates regenerating rice calli?

I am regenerating transformed rice calli on MS-medium. Before this, all stages were grown in the dark. But after keeping them in light for regeneration, a lot of condensation has formed on the lid...

12 October 2014 8,347 16 View

Does anyone have experience deciding parameters for RNA-seq on Arabidopsis?

I have a question for doing RNA-seq on Arabidopsis samples comparing differential gene expression between wild type and miRNA mutant lines. Just one mutant will be compared.How many replicates are...

22 August 2014 345 1 View