Has anyone had weird polybrene toxicity during viral transduction?

Hi, I'm transducing Ly1 and L363 cell lines using our standard protocol for retroviral transduction. The cells are successfully transduced as evidenced by GFP expression. However, after 4-5 days...

26 January 2021 4,071 3 View

How can I find metals and alloys that have specific Vickers hardnesses?

I need to find metals, plastics or metal alloys that have a specific Vickers scale hardness scores. I'm not aware of any existing web sites and tools that could aid me in searching for this data,...

06 August 2020 4,842 4 View

How to adjust dependent variable to make it usable?

Hello, We're conducting quantitative research as a part of this semester, and the analysis method of choice is multivariate linear regression. Right now, we're checking for the fulfillment of...

03 November 2019 2,694 3 View

How to express sex ratio when the number of females is 0?

In general, sex ratio is expressed as the ratio of males to females in a population, ie number of males/number of females (*100). However, when the number of females in (sub)population is 0...

18 August 2019 1,856 28 View

Which WB STAT3 and STAT5 antibodies are best?

Hi, I'm currently having problems with Western blots using the following antibodies from Cell Signaling: Phospho-Stat3 (Tyr705) STAT3 (total) Phospho-Stat5 (Tyr694) STAT5 (total) They seem to...

11 August 2019 3,463 4 View

Does PLE and PL have different sensitivity to detect defects in solid state?

Since using PLE carriers can be excited to the bottom of the conduction band, where they kinetic energy is zero it should be less probable to detect luminescence coming from carriers influenced by...

10 July 2019 3,335 3 View

Combining Dharmacon siRNA+Lipofectamine?

Can I transfect HEK293 cells using siRNA purchased form Dharmacon and use Lipofectamine reagent instead of DharmaFECT? I have been using electroporation for the transfection, but it seems like HEK...

21 January 2019 2,884 3 View

Overlap Extension PCR for closed plasmids?

Hi, I want to do an overlap extension PCR (OE-PCR) using a PCR-amplified plasmid backbone and a PCR-amplified insert. The OE-PCR shall add the insert into the plasmid similar to this...

12 July 2018 1,976 3 View

Is CDDQ (Career Decision-making Difficulties Questionnaire) usable for university students (22-24 years old)?

I am from Czechia (in past Czechoslovakia). I do research to check if the problems in choosing a field of study (measurred by CDDQ) are related to MBTI typology dimensions. I know this method is...

28 April 2018 9,264 1 View

Experiences of liquid handling system for qPCR and NGS library preparation (amplicon seq)?

We are looking into liquid handling systems for pipetting qPCR plates (384 well) and for NGS library preps (amplicon seq). There are many machines on the market that are able to do that (Biomek...

25 February 2018 8,701 5 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

How to include original NMR data in a manuscript?

hello. i am junior researcher. I received a comment from a reviewer that he could not find NMR data in my submitted paper. I had included the NMR graph with explanation and formula to calculate...

27 February 2021 734 4 View

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View

When I reacted Bidentate NS Schiff Base with metal salt (M-Cl2) in +2 oxidation state in proportion of (2:1) , getting sticky product ,, why?

When I reacted Bidentate NS Schiff Base with metal salt (M-Cl2) metal chlorides in +2 oxidation state in proportion of (2:1 Schiff base &metal salt) , i was get sticky material Why ? ---~...

21 February 2021 7,970 5 View

What is the actual chemistry going on between iodate and ascorbic acid?

I am studying biochemistry at university, as part of the course we do several titration experiments. In our handouts, we were given the following half equations: IO3- + 6H+ + 4e- I+ +...

16 February 2021 8,731 1 View

Acid phenol:chloroform (25:24:1) for RNA extraction instead of (125:24:1)?

Hi everyone! I am about to extract RNA for RNA sequencing assays and my Invitrogen kit/protocol...

14 February 2021 1,755 3 View