7 Questions 28 Answers 0 Followers
Questions related from Filip Garbicz
Hi, I'm transducing Ly1 and L363 cell lines using our standard protocol for retroviral transduction. The cells are successfully transduced as evidenced by GFP expression. However, after 4-5 days...
27 January 2021 4,272 3 View
Hi, I'm currently having problems with Western blots using the following antibodies from Cell Signaling: Phospho-Stat3 (Tyr705) STAT3 (total) Phospho-Stat5 (Tyr694) STAT5 (total) They seem to...
12 August 2019 3,585 4 View
A recent paper searching for novel mutations using whole-exome sequencing with preamplification assumed the following thresholds:> 6 (and 20> 10%...
07 April 2015 3,665 6 View
I have found a mutation that probably decreases the level of transcription of my gene of interest. I would like to clone a part of the gene into a plasmid that would contain a reporter gene, so...
01 November 2014 1,541 8 View
I would like to measure the activity of FAS/TNFR-dependent pathways and apoptosome-dependent pathways separately using FACS or a fluorometer. I do NOT want to use Western blot. The best solution...
28 March 2014 4,878 17 View
Not having much experience with sequencing, I would like somebody to look at the attachment. According to the sequence results, there is an insertion between 68G and 70A. That cytosine peak is not...
01 March 2014 672 26 View
There is a major problem with transfections in our project. I have recently tested Lipofectamine 2000 and a Cyan Fluorescent Protein (CFP) plasmid on CHO adherent cells. According to the protocol,...
28 February 2014 10,075 25 View