Filip Garbicz

7 Questions 27 Answers 0 Followers

Questions related from Filip Garbicz

Has anyone had weird polybrene toxicity during viral transduction?

Hi, I'm transducing Ly1 and L363 cell lines using our standard protocol for retroviral transduction. The cells are successfully transduced as evidenced by GFP expression. However, after 4-5 days...

27 January 2021 4,246 3 View

Which WB STAT3 and STAT5 antibodies are best?

Hi, I'm currently having problems with Western blots using the following antibodies from Cell Signaling: Phospho-Stat3 (Tyr705) STAT3 (total) Phospho-Stat5 (Tyr694) STAT5 (total) They seem to...

12 August 2019 3,566 4 View

Does anyone have suggestions on NGS Whole-exome sequencing - controversial thresholds?

A recent paper searching for novel mutations using whole-exome sequencing with preamplification assumed the following thresholds:> 6 (and 20> 10%...

07 April 2015 3,654 6 View

Which reporter plasmid do you recommend for mutated gene transcription analysis?

I have found a mutation that probably decreases the level of transcription of my gene of interest. I would like to clone a part of the gene into a plasmid that would contain a reporter gene, so...

01 November 2014 1,529 8 View

How can you measure the activity of ONLY extrinsic or ONLY intrinsic pathways in apoptosis?

I would like to measure the activity of FAS/TNFR-dependent pathways and apoptosome-dependent pathways separately using FACS or a fluorometer. I do NOT want to use Western blot. The best solution...

28 March 2014 4,870 17 View

Can anyone verify whether this is an artifact or an insertion in these Sanger sequencing results?

Not having much experience with sequencing, I would like somebody to look at the attachment. According to the sequence results, there is an insertion between 68G and 70A. That cytosine peak is not...

01 March 2014 658 26 View

Has anyone experienced very low transfection efficiency using Lipofectamine 2000?

There is a major problem with transfections in our project. I have recently tested Lipofectamine 2000 and a Cyan Fluorescent Protein (CFP) plasmid on CHO adherent cells. According to the protocol,...

28 February 2014 10,060 25 View