Has anyone had weird polybrene toxicity during viral transduction?

Hi, I'm transducing Ly1 and L363 cell lines using our standard protocol for retroviral transduction. The cells are successfully transduced as evidenced by GFP expression. However, after 4-5 days...

26 January 2021 4,071 3 View

How can I find metals and alloys that have specific Vickers hardnesses?

I need to find metals, plastics or metal alloys that have a specific Vickers scale hardness scores. I'm not aware of any existing web sites and tools that could aid me in searching for this data,...

06 August 2020 4,842 4 View

How to adjust dependent variable to make it usable?

Hello, We're conducting quantitative research as a part of this semester, and the analysis method of choice is multivariate linear regression. Right now, we're checking for the fulfillment of...

03 November 2019 2,694 3 View

How to express sex ratio when the number of females is 0?

In general, sex ratio is expressed as the ratio of males to females in a population, ie number of males/number of females (*100). However, when the number of females in (sub)population is 0...

18 August 2019 1,856 28 View

Which WB STAT3 and STAT5 antibodies are best?

Hi, I'm currently having problems with Western blots using the following antibodies from Cell Signaling: Phospho-Stat3 (Tyr705) STAT3 (total) Phospho-Stat5 (Tyr694) STAT5 (total) They seem to...

11 August 2019 3,463 4 View

Does PLE and PL have different sensitivity to detect defects in solid state?

Since using PLE carriers can be excited to the bottom of the conduction band, where they kinetic energy is zero it should be less probable to detect luminescence coming from carriers influenced by...

10 July 2019 3,335 3 View

Combining Dharmacon siRNA+Lipofectamine?

Can I transfect HEK293 cells using siRNA purchased form Dharmacon and use Lipofectamine reagent instead of DharmaFECT? I have been using electroporation for the transfection, but it seems like HEK...

21 January 2019 2,884 3 View

Overlap Extension PCR for closed plasmids?

Hi, I want to do an overlap extension PCR (OE-PCR) using a PCR-amplified plasmid backbone and a PCR-amplified insert. The OE-PCR shall add the insert into the plasmid similar to this...

12 July 2018 1,976 3 View

Is CDDQ (Career Decision-making Difficulties Questionnaire) usable for university students (22-24 years old)?

I am from Czechia (in past Czechoslovakia). I do research to check if the problems in choosing a field of study (measurred by CDDQ) are related to MBTI typology dimensions. I know this method is...

28 April 2018 9,264 1 View

Experiences of liquid handling system for qPCR and NGS library preparation (amplicon seq)?

We are looking into liquid handling systems for pipetting qPCR plates (384 well) and for NGS library preps (amplicon seq). There are many machines on the market that are able to do that (Biomek...

25 February 2018 8,701 5 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

How climate change will influences human and animal behavioral change?

The role of climate change influencing the human behavior as well as economics interest

01 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View

Good sites to recruit survey participants on a grad school student budget?

Any good recommendations for participant recruitment for online surveys on a grad school student budget?

02 March 2021 4,293 4 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View