Both forward and reverse?
sine your vector contains T7 promotor before the MCS, you can use T7 forward primer for sequencing.
No need of reverse for this? @sansrity sinha
Hi there,
As the plasmid contains both T7 promoter and T7 terminator you may use T7 pro and T7 ter primer set.
Nucleic acids
04 May 2018 2,910 0 View
04 May 2018 8,103 0 View
So that it can be stored in below 80 degree conditions.
01 February 2018 669 3 View
Blood paramters
31 December 2017 2,159 2 View
Also, I have come across NK cell whole blood count & percentage wise count. Which is a better option if I need to use it as tumour biomarker?
04 May 2017 9,400 3 View
With refrences
04 May 2017 9,666 0 View
For cloning purpose.
04 May 2016 8,555 0 View
Protein expression vector
04 May 2016 2,854 1 View
After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...
08 August 2024 1,668 3 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
Please can anyone support with the survey questions based on RQ measures and propose how to do it in FMCG industry and include as well the role of brand equity Thanks
06 August 2024 949 0 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
How to design VN primer to attach with universal reverse primer
05 August 2024 2,116 3 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View