For cloning purpose.
Nucleic acids
04 May 2018 2,910 0 View
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So that it can be stored in below 80 degree conditions.
01 February 2018 669 3 View
Blood paramters
31 December 2017 2,159 2 View
Also, I have come across NK cell whole blood count & percentage wise count. Which is a better option if I need to use it as tumour biomarker?
04 May 2017 9,400 3 View
With refrences
04 May 2017 9,666 0 View
Both forward and reverse?
06 July 2016 6,699 3 View
Protein expression vector
04 May 2016 2,854 1 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
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I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
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Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
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i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
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Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
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Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
Hi, everyone. I want to transfer two genes in to the pseudomonas bacteria that isolated from the soil. The bacteria that I want to use for cloning haven't been identified and not be sequenced,...
02 August 2024 3,987 1 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
I'm fairly new to making homemade comp cells and have done so with some mild success. What is generally an acceptable way of resuspending comp cells during wash steps? I've been told to swirl the...
31 July 2024 8,309 0 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
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