How to separate two close peak and peak tailing in HPLC?

07 August 2017 3,484 23 View

Which is the best method for estimation of total Saponin from Aloe vera Using HPLC?

07 August 2015 8,911 7 View

Which solutions/chemicals should be used to remove the polyacrylamide gel from glass plates?

I am using Biorad sequi gen sequencing gel apparatus for AFLP. In which one a plate is treated with Bind silane. After staining this is very difficult to remove the gel from the glass plate....

05 June 2014 4,901 4 View

How do I design the SSR markers from NGS data?

We have sequenced the plant transcriptome using next generation sequencing. Now we have to Design SSR markers from this data. Please suggest how to design these markers.

03 April 2014 1,532 5 View

Can someone suggest the best suited DNA isolation method for many Fungal strains?

Seeking a suggestion of any DNA isolation method for different fungal strains. Some methods are working for particular strains. But I want a method which works for different strains and also best...

07 August 2013 3,288 1 View

Can someone suggest a HPLC Method for the extraction and estimation of saponin from plants?

Please suggest HPLC method for estimation of saponin. Extraction method? estimation method? HPLC column?

06 July 2013 5,983 3 View

Problem with the cDNA purity in RNA extraction by LiCl method

I have synthesized cDNA from RNA (extracted by LiCl method) using Thermo RevertAid cDNA synthesis kit. The 260/280 ratio for all the cDNA samples is falling in the range 1.6-1.7 while it should be...

05 June 2013 2,124 5 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

No title

02 March 2021 3,060 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View