23 February 2023 1 7K Report

Currently, I am working with yeast. I am treating it with different doses of radioactivity. I want to see if the DNA shows foci such as Gamma H2AX.

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Beta galactosidase assay in milk?

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How to conduct/interpret power analysis for binary GLMM within-subjects with 2x3 independent factors?

Dear community, I can't seem to find any clear information on power analyses work so I'm throwing my question out onto the internet hoping for answers. :) Study Details I am doing a...

19 May 2024 9,214 4 View

Does TSO- salt in a 5% NaHCO3 solution turn the solution into a dark green color?

Here's the brief: 1. I synthesized alkyl gallates (ethyl gallate, butyl gallate, and amyl gallate) using Fischer esterification. 2. I used TsOH instead of concentrated H2SO4 as the catalyst. 3....

03 May 2024 6,737 5 View

Help on Modified Nanopore Sequencing to do Sanger-like sequencing for PCR amplicon from bisulfite converted DNA?

Hi, I'm planning to do sequencing using Nanopore. The main idea is to substitute Sanger Sequencing that usually used to sequence PCR amplicon from BCD (Bisulfite Converted DNA) using nanopore...

23 April 2024 6,637 0 View

Lyophilization of leaves, stem bark, fruits?

Is it recommended to freeze dry harvested leaves, stem bark, and fruits prior to trituration and extraction? I'll use the extracts for phytochemical analysis & in vitro bioassays. Thank you...

31 March 2024 5,920 4 View

Are nature-based solutions relevant to building a resilient society in the face of drinking water scarcity?

Although there have been a number of initiatives, the shortage of drinking water is still a problem for some communities. In this context, we can see that people's need for water is increasing as...

26 February 2024 5,285 3 View

How to remove DCU and its remaining traces from Steglich esterification?

Firstly, the compounds that I want to synthesize are alkyl gallates (ethyl gallate, butyl gallate, and hexyl gallate). The overall reaction: Gallic Acid + Alcohols + (THF, DCC, DMAP, 0 Celsius)...

25 February 2024 2,181 3 View

Has anyone worked with Science Paths - reportedly a book writing service?

Hello everyone, I receive many emails about different journals and publishers inviting manuscripts (some predatory, some not); however, received something new. Someone from Science Paths sent an...

12 December 2023 1,819 0 View

Achieving the SDGs by 2030: myth or reality?

According to a report by the Sustainable Development Solutions Network (SDSN) published in 2023, only 18% of SDG targets will be met by 2030. Should we expect a new global agenda?

26 November 2023 7,921 3 View

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