Lipofectamine 2000

You can try HighperFect transfection reagent (Qiagen)

Dear Naoto,

Transfecting primary macrophages is really, really challenging, and so far the only efficient method is the viral one.

Nevertheless, depending on the application and the molecule to deliver, it is true that some relatively nice results can be achieved by classical transfection reagents.

For siRNA, I would recommend you two alternatives :

• SilenceMag, using the Magnetofection technology*** was demonstrated to be highly efficient for siRNA transfection into Macrophages to study endocytic trafficking (Kadiu I., Nanomedicine, 2011: http://www.ncbi.nlm.nih.gov/pubmed/21417829)
• DreamFect, that is a lipid-based reagent usually dedicated to DNA transfection but that was used to deliver siRNA into monocytic suspension cells (Fleischmann KK., Mol Cancer. 2014: http://www.ncbi.nlm.nih.gov/pubmed/24517546).

Would you like to try these reagents, please do not hesitate to contact me at: [email protected]

good luck with your experiment,

best regards,

Cedric

*** The Magnetofection technology uses a magnetic field to attract and concentrate complexes of magnetic nanoparticles and nucleic acid onto the cell surface as demonstrated by Grześkowiak BF et al, Pharm Res. 2014 Jul 18. (http://www.ncbi.nlm.nih.gov/pubmed/25033763).

http://www.ozbiosciences.com/content/13-magnetofection

Thank you everyone. I would try one by one.

Hi Naoto,

for transfection of siRNA in primary macrophages I use a new transfection reagent called Viromer RED.

Its a synthetic polymer zero in charge. Due to an active endosome escape mechanism I get higher transfection efficiency compared to standard reagents:

https://www.ssl-id.de/www.lipocalyx.de/applications/plasmid-data/?force_sid=2o19o35iie9rs7ib8rja094ak1