I want to establish the high efficient cardiomyocyte differentiation system using mouse embryonic stem cell (mESCs). My colleague told me the hanging drop method (old and classical but famous one). This worked well a little bit but it was not enough efficiency (around 1-5%) to conduct further studies.
I want to research the stress response of mESC-derived cardiomyocyte. It needs more purity and mass. If you know other better methods, please tell me.
Now I'm planning to try the two following methods; glucose-depleted lactate-added culture (Shugo Tohyama, 2012) and Noggin treatment (Yuasa S, 2005).
You can refer to the links below:-
1. http://www.lifetechnologies.com/in/en/home/life-science/stem-cell-research/stem-cell-differentiation/culture-systems-reagents-cardiomyocyte-differentiation.html
2. https://www.rndsystems.com/products/stemxvivo-cardiomyocyte-differentiation-kit_sc032
3. http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0018293
4. http://www.ncbi.nlm.nih.gov/pubmed/24667642
Dear Naoto,
I would strongly recommend you to try out this protocol:
http://www.ncbi.nlm.nih.gov/pubmed/23257984
It works very nice with both human iPSCs and embryonic stem cells and it is quite easy and fast.
Good Luck!
Dear Sandip, Holger, Elphège, and Stefania
I really appreciate you!
Most of them are informative and useful, especially bmp4 & activin A treatments. As I use "mouse" ES cells which are mostly different from human ESC, the established method is just required!
Other methods are also interesting. When the bmp4 method doesn't work I will try them.
Anyway, thank you very much!
Hi Naoto,
Did you succeed? Which protocol was the best? I have an expertise with human cardio protocols and now have to do it with mouse ESCs. I found one "no EBs" protocol. This is the reference: Dev Dyn. 2016 Feb;245(2):157-65. doi: 10.1002/dvdy.24366. Did you try it by chance?
Elena
Dear Naoto Noma,
Do you get any good high efficient cardiomyocyte differentiation system using mouse embryonic stem cell (mESCs)?I am doing this experiment now.Thank you.
Best wishes.
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