Hello Shreya

Neurons are sensitive to cytotoxicity and difficult to transfect with most of the methods that are used. As you have already mentioned I suggest you should go for Magnetofection which is a novel transfection technology based on magnetic nanobeads. It is efficient and non-toxic.

Please refer to the following research articles for more information.

https://pubmed.ncbi.nlm.nih.gov/18079708/

Article High-efficiency transfection of cultured primary motor neuro...

Best Wishes.

Dear Malcolm,

Many thanks for your answer and support.

I look forward using magnetofection to tranfect primary hippocampal neurons.

Thanks once again.@Malcolm Nobre

Hello Shreya,

Primary neurons are really sensitive and easily to get died. For my research I use lentivirus transfection depend on specific experimental design. But would like hear from you if you succeed using magnetofectin.

Thank you