Which tool would you use to draw a linear genome map for Staphylococci SCCmec?

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Is piranha solution or oxygen plasma more effective for cleaning gold surfaces?

I am generating self assembling monolayers of thiol-modified DNA strands on a gold surface, and I'd like to clean and reuse the gold surfaces. From what I've seen, folks typically use either...

17 November 2023 5,331 1 View

How do I make a 6-mercapto-1-hexanol (MCH) working dilution?

I am using 6-mercapto-1-hexanol (MCH) as part of the immobilization procedure for forming a self-assembled DNA monolayer on a gold surface (see the first figure of DOI: 10.1002/celc.201402273 for...

27 April 2023 2,451 0 View

Methods for immobilization of DNA on gold surfaces?

I've been looking for methods for immobilizing 5'-Thiol modified dsDNA on gold slides. Most of what I've come across suggests using DTT followed by a desalting step or using TCEP. However, I...

02 April 2023 1,680 5 View

How can I compare the path of 2 spherical pendulums in 3D space?

I am trying to plot and analyze the difference (or similarities) between the path of two spherical pendulums over time. I have Cartesian (X/Y/Z) coordinates from an accelerometer/gyroscope...

16 November 2022 6,675 2 View

Is ABAQUS able to output what I need for Fatigue Analysis in MATLAB?

I am wanting to apply the hot spot stress approach to an offshore jacket structure. This involves find the nominal stress at joint intersections, forces and moments. I am struggling to output the...

16 March 2018 4,667 1 View

Can anyone help with the strange correlation between particle count and speed in flow cytometry?

I am counting emulsion droplets using flow cytometry and I have noticed that the final volumetric count of the particles depends heavily on the speed. A higher flow rate results in a higher final...

16 July 2014 5,309 9 View

Can anyone help with Peg-Biotin and particle aggregation (agglutination)?

I am trying to aggregate particles functionalized with biotin using streptavidin. Is inter-particle aggregation still possible if the biotin is at the end of a peg polymer chain? (for example peg...

15 June 2014 3,079 3 View

Does anyone know of any publications already available looking into the effect of sugar solution on the growth of young tree stock?

I am looking into the effect of different growth media on the successful establishment of young root stock. Due to this I am looking into previous studies on the different growth medias (i.e....

20 February 2014 7,520 4 View

Does anyone know of a validated questionnaire examining social isolation and or homophobia that I could access?

The Liverpool Mental Health Consortium LGBT group are planning on administering a questionnaire to the LGBT Liverpool community

08 January 2014 4,306 2 View

Does anybody have any advice about the formation of liposomes and micelles in phospholipid emulsions?

If I make an oil-in-water emulsion using a phospholipid as the surfactant (which begins dissolved in the oil phase), will I always have some liposomes and micelles present too? If so are there any...

25 November 2013 5,563 2 View

How can we use mobile apps for improving students' academic performance?

Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance

04 August 2024 9,492 0 View

Do you know best conditions for formation of best REE deposits?

I want to know more about REE deposits.

03 August 2024 6,930 2 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

How to draw the five chelate rings of [Co(EDTA)]- on this picture?

Is this circle one of the rings?

03 August 2024 5,988 0 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

What is the best conditions for formation of REE ore deposits?

I want to know more about REE deposits.

31 July 2024 7,366 1 View

How to make overlapping p-h diagram in EES?

I want to draw ph diagram using Engineering equation solver EES for refrigerant R134a and R1234fy and some other. I don't know how to draw the multiple ph diagram on same dome or overlapping to...

30 July 2024 647 7 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View

How can I get old refernce in geomorphological mapping?

how can I read Waters, R. S. 1958. Morphological mapping. Geography 43 :10-17 from internet? note: not in google or resaearch gate

26 July 2024 7,813 3 View

How to merge two or more linkage maps?

I have three linkage maps that needs to be integrated to create a consensus map for QTL mapping.

25 July 2024 6,505 2 View