I am using 6-mercapto-1-hexanol (MCH) as part of the immobilization procedure for forming a self-assembled DNA monolayer on a gold surface (see the first figure of DOI: 10.1002/celc.201402273 for a good visual representation). Most protocols I see online and in the literature say to use aqueous 1 mM MCH, but I am struggling to make this working dilution. As far as I know, MCH always comes as a liquid (I ordered mine from TCI chemicals, Ref# M2266). I tried first making a stock 10 mM solution by mixing 1 mg of MCH liquid with 745 µL of water. Unfortunately, the MCH appeared to be immiscible in water, forming distinct droplets in the water that rise to the top without mixing.
How do I make a working or stock dilution of MCH in water that isn't biphasic?
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