Does anyone know of a validated questionnaire examining social isolation and or homophobia that I could access?

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Is piranha solution or oxygen plasma more effective for cleaning gold surfaces?

I am generating self assembling monolayers of thiol-modified DNA strands on a gold surface, and I'd like to clean and reuse the gold surfaces. From what I've seen, folks typically use either...

17 November 2023 5,331 1 View

How do I make a 6-mercapto-1-hexanol (MCH) working dilution?

I am using 6-mercapto-1-hexanol (MCH) as part of the immobilization procedure for forming a self-assembled DNA monolayer on a gold surface (see the first figure of DOI: 10.1002/celc.201402273 for...

27 April 2023 2,451 0 View

Methods for immobilization of DNA on gold surfaces?

I've been looking for methods for immobilizing 5'-Thiol modified dsDNA on gold slides. Most of what I've come across suggests using DTT followed by a desalting step or using TCEP. However, I...

02 April 2023 1,680 5 View

How can I compare the path of 2 spherical pendulums in 3D space?

I am trying to plot and analyze the difference (or similarities) between the path of two spherical pendulums over time. I have Cartesian (X/Y/Z) coordinates from an accelerometer/gyroscope...

16 November 2022 6,675 2 View

Any research on modeling skew Gaussian spatial data in the presence of censored data?

15 December 2018 4,443 3 View

Outliers, keep or delete?

Hi everyone, I have been given an assignment for uni and I am not sure whether to keep or remove outliers. After running normality assumptions, the data reported to be normally distributed,...

10 May 2018 6,743 4 View

Is ABAQUS able to output what I need for Fatigue Analysis in MATLAB?

I am wanting to apply the hot spot stress approach to an offshore jacket structure. This involves find the nominal stress at joint intersections, forces and moments. I am struggling to output the...

16 March 2018 4,667 1 View

How could alanine substitution vs deletion have an impact on a protein?

I performed deletion mutagenesis along the c terminal region of a protein targeting two specific leucine residues, and showed no change compared to wild type, as assayed by dual...

05 February 2018 4,507 2 View

Redundant non-target deletion next to desired mutation site? (PCR site-directed mutagenesis)

Hello, I am conducting site directed deletion mutagenesis of pcDNA5 and I am acquiring my desired mutations, however, there is always an additional one or two codons deleted following my desired...

20 August 2017 8,133 2 View

How do I use Q-Methodology?

Hello everyone, I am about to embark on my first research project for my dissertation, my method of research I intend to use is Q-methodology, I am just learning all the nuances of this form of...

26 April 2015 4,123 10 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Is there an alternative to a multinomial regression which allows the DV to be non mutually exclusive?

I am trying to analyse data from a survey examining what variables affect teachers perceived barriers to incorporating technology into their classroom. I have 5 predictor variables however my DV...

06 August 2024 1,752 3 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

Why do exism movements become permanent dictatorship threats within liberal democracy thinking under majority rule-independent rule of law system?

Exism movements after gaining power within liberal democracies under majority rule and independent rule of law system become permanent dictatorship threats, but why this is the case is not clear...

04 August 2024 8,125 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How Social Media Affects Your Mental Health ?

How Social Media Affects Your Mental Health

04 August 2024 6,961 3 View

I need the datasets of Microgrid for system identification?

Hi I am working on data driven model of the microgrid, for that, i need the reliable datasets for the identification of MG data driven Model. Thanks

02 August 2024 5,748 4 View

Are current regulations effective in preventing cancer caused by toxins?

I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from experts in the field. If you are an expert or researcher working on...

02 August 2024 4,474 2 View

Should I remove an item from a scale to raise Cronbach's alpha and McDonald's omega or is it better to leave it if they are both over .7 already?

Hello! I have this scale which had 10 items initially. I had to remove items 8 and 10 because they correlated negatively with the scale, and then I removed item 9 because Cronbach's alpha and...

01 August 2024 4,606 7 View