Which tool I can use to build miRNA-RNA interaction?

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Why the analytical method is better for evaluating the cloud? and what is the difference between simulation and analytical methods?

Hello every one, in several articles about"performance evaluation in cloud computing," authors announced that the analytical method is the most suitable for evaluating the cloud system, I want to...

22 October 2020 1,573 3 View

How we choose the most suitable analytical modeling technique for cloud computing?

Accurate performance evaluation of Cloud Computing resources is a necessary prerequisite for both client and provider to ensure that quality of service parameters remain within agreed limits. For...

15 October 2020 6,819 4 View

How can I show values of significance in a bargraph?

Hi, Please, is there anyone who knows how can I demonstrate p values (or asteriks of significance) on a bargraph. I need Free download softwares. Thank you.

21 February 2020 8,590 2 View

How to remove the solvent box from the em.pdb file after energy minimization in GROMACS?

Greetings all, I converted the .gro file to a .pdb file using the command: gmx editconf -f em.gro -o em.pdb To validate the minimized energy structure and calculate the RMSD using pymol, I need...

15 January 2020 9,151 4 View

How to analyze ranks and scores in SPSS?

What statistical analyses/tests are available in SPSS to analyze (partial and full) RANKING data and RATING data?

01 January 1970 976 5 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Can MicroRNA cross the blood brain barrier?

In diseased conditions, many genes and gene products are visible in the bloodstream. So my question is whether we can find traces of microRNA which is involved in disease and disorder in blood serum?

24 February 2021 7,064 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View

Has anyone encountered any problems with abm good microRNA primers for quantification of mature miRNAs?

I've been trying to quantitate mature microRNAs in 293T cells with and without 'X'. 2ug of total RNA (isolated by Trizol method) is converted to cDNA using All-in one microRNA cDNA synthesis kit...

17 February 2021 8,986 2 View

What is the best way to fix plasma derived miRNA for subsequent cDNA synthesis?

I need to quantify blood plasma derived miRNA through q-RT-PCR. For this I need to fix RNA content in my cDNA reaction. What is the best way to do this? Currently, I take readings of my plasma...

03 February 2021 9,330 3 View

What is the general miRNA yield one gets from the Qiagen miRNEASY serum/plasma advanced kit?

I am using the Qiagen miRNEASY serum/plasma advanced kit, using 400ul of frozen plasma. What is the yield you get on average? I am using nanodrop currently for quantification. I am getting...

02 February 2021 8,806 2 View

Which miRNA arm is a right target for analysis, 3p or 5p?

I'm working on selecting mouse miRNAs to analyze and stumbling upon one confusing issue, some of the papers do not specify which arm they analyzed and they just state something like " miR-34a was...

16 January 2021 3,792 4 View

Is negative controls necessary for western blotting?

I am trying to confirm my miRNA target by western blotting. I have a ds mimic and a ss inhibitor of my miRNA. Is it necessary to use negative controls for mimic and inhibitor? If yes, how can I...

09 January 2021 2,320 3 View

Can I predict miRNA by knowing the mRNA target?

Hello! I am trying to predict in silico the miRNA by knowing the target. Most of published research starts by knowing the miRNA and predicts the target using online tools. Are you familiar with...

31 December 2020 7,433 5 View

What are the possible drawbacks of using miRNA sponge to enhance the specificity of gene expression?

I am searching for miRNA repeats put between WPRE and 3'LTR of my lentivirus, in order to enhance the specificity of transgene expression in human neuron cells. But if clinically translation of...

26 December 2020 9,921 2 View