Hi,
Can anyone suggest the potential hexose and pentose that can be a carbon source for Pseudomonas putida KT2440 or F1? And their metabolism maps?
The one I know is glucose and fructose. Any else?
Thanks.
If my memory serves me correctly, Ps putida will grow on most monosaccharides. There is a paper from the Stanier group at Berkeley from 1965 ( with Norberto Palleroni) which covers all aspects of Pseudomonas sp. The journal is J. Gen. Microbiol.
Hi, Bhoj. Really nice of you. It is very useful.
I am working on putida strains, and just wandering if you can share any more details of putida, like which strain can or cannot go with specific sugars. There are only numbers in your xlsx file, but if you can share me with the detailed names of strains, that will be really great.
to whom following this question, I did a growth test of several sugars with p.putida F1, what i can confirm now is: from single colonies, cells can grow on all sugars I tested, but only fructose, lactose can lead to a reasonable cell density; maximum cell densities are all below 0.2(OD600) for other sugars, including D-maltose, D-galactose, L-arabinose, D-sorbital, sucrose, L-Rhamnose, Myo-inositol. And the lag phase for all sugars tested are around 17-20h, while it will be only few hours with glucose. The medium I used is a defined mineral medium.
- Badges
- Science topic