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Questions related from Bin Lai
Dears, Is there any one having any experience with knocking out tonB gene, to disrupt TonB dependent transporting system in bacterial? we constructed the knockout plasmid but could not get any...
19 September 2018 8,597 3 View
Hi, I have been noticed a phenomenon for long that if the ferricyanide solution (in salt buffer) was left open on the bench, the solution would become colorless in hours; but if the solution is...
04 May 2018 5,032 1 View
I currently come to a question about the buffer pH for bacteria protein extraction. I read some papers (also for my own protocols) and they always have a lysis buffer at e.g. pH 7,4-7,6, and I am...
21 February 2018 3,229 9 View
Could anyone suggest any bacterial friendly method to completely remove the oxygen from the medium for a single cell analysis system? flushing with nitrogen is not good enough and we could not put...
30 November 2017 6,955 5 View
recently, I was trying to get a kinetic view of the reaction rate within the biofilm and on electrode surface. Based on Nicholson's equation, k0 of a redox reaction on electrode surface could be...
06 December 2016 592 8 View
Two issues about membrane protein analysis want to be addressed here: 1. after sodium carbonate extraction, will you collect the supernatant for protein precipitation? if yes, how did you do that...
22 October 2015 8,044 8 View
Hi, I noticed it's a bit hard to collect the membrane fractions after a sucrose gradient ultra-centrifugation. Does anyone have a rich experience in it and have any "secret tips" to collect them...
14 August 2015 6,642 9 View
Hi, Can anyone suggest the potential hexose and pentose that can be a carbon source for Pseudomonas putida KT2440 or F1? And their metabolism maps? The one I know is glucose and fructose. Any...
17 June 2015 2,692 4 View
We tried to do those measurements with a commercial colorimetric assay kit, but finally found its sensitivity does not fit our case. That can only give accurate readings for 10pmol changes which...
18 November 2014 926 12 View
Does anyone know the solubility or have ever done solubility tests of NADH and NADPH in pure DMSO? If soluble, what form are they in, tetra salt?
19 October 2014 6,403 10 View
