I'm working on a polymer with NIPAAm and n,n-bis(acryloyl)cystamine ( molar ratio ~ 15:1 ), and I want to reduce the disulfide into thiol group. I used TCEP dissolved in PBS and adjust the pH ~ 7, but after I mixed it with my polymer, it precipitated and I don't know why. I tried changing the temp. but it didn't work and I don't know why? I also tried DTT but the same situation happened.
The polymer was precipitated by ether, is it possible that it's because I didn't dried the polymer under vaccum long enough?
Thanks for ur time~