I am interested to know how can I modeling Troposphere with Bernese software? It means that can you introduce a source for it?

I readed the Bernese manual which that published at Bernese website, but it was'nt enough.

10 March 2020 6,098 3 View

Have you ever seen that 40S and 60S of ribosome subunit peaks disappear from polysome fractionation?

I am doing polysome fractionation for stress conditions for mammalian cell. it seems that peaks of 40S and 60S disappear during stress condition compared to control. I am wondering whether all...

12 November 2018 3,513 2 View

How is 3D pelvic kinematics examined during walking before and after interventional exercises in patients with pelvic inclination?

in general, for healthy people, in the 3D motion analysis to define the anatomical coordinate system on segments, one frame in static position is taken by the camera, which will make segment...

10 October 2018 4,098 2 View

Has anybody transfected an integrative plasmid DNA into rat bone marrow-derived mesenchymal stem cells by lipofectamine 2000 with a high efficiency?

I am working with rat BM-MSCs, and i am trying to transfect these cell with integrative pIRES-EGFP plasmid by applying lipofectamine 2000 (invitrogen). however I found these cells very hard to...

31 August 2018 9,851 3 View

How residential satisfaction and sustainability could be related to each other?

i need some references which have discussed how residential satisfaction may lead to different aspects of sustainability.

20 June 2018 7,713 3 View

Does anyone know how to scoring Three factor eating-18 questionnaire?

I have some question about questionnaire scoring. I would be grateful if anyone kindly guides me in these questions. My questions are:If The response Likert for questions 14 to 17 are different...

25 September 2017 5,040 3 View

What is the best solvent to dissolve Perylene-d12?

30 August 2017 1,493 1 View

What is the reason for change in mRNA level of specific gene but not change in protein level?

With treatment of cell lines ( cardiofibroblast cells), changes in mRNA level of the gene of interest has been observed overtly, but there is no change in protein level. I am wondering how this...

19 August 2017 1,026 6 View

Cooper nanoparticle

28 December 2016 7,410 6 View

Cooper nanoparticle

This is UV peak and change color

28 December 2016 6,082 2 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Can the problems of overfitting be solved without cross validation?

For an overfit data set the cross validation error will be much bigger than the training error. Is it true or false?

23 February 2021 3,616 2 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

How to manage western blot for proteomics data validation?

Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...

23 February 2021 6,671 4 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View