Dear Fereshteh Jeivad

You can measure the activity of NF-kB by checking expression of I kappa B-alpha mRNA using RT-PCR

Article Monitoring NF-κB Transactivation Potential Via Real-Time PCR...

Or by western blot you can check phospho-P65

Hope this would be useful.

Best regards

Isehaq

Hello,

I would suggest you directly go for NF-kB activity assay or you can do western blotting for phospho-p65.

Cheeers.....