Isehaq Al-Huseini

7 Questions 21 Answers 0 Followers

Questions related from Isehaq Al-Huseini

How many passages can we keep neonatal cardiomyocytes?

Is anyone familiar with culturing of neonatal cardiomyocytes? I have successfuly isolated and cultured primary neonatal cardiomyocytes from new born mice (2 days old), I would like to know how...

25 April 2017 9,373 7 View

What is the recommended maximum passage number for primary vascular smooth muscle cells (VSMCs)?

I am using primary VSMCs isolated from Abdominal aorta of knockout and transgenic mice. After passage 10, I noticed some changes in prolifration rate and expression of some protein. What is the...

03 November 2016 9,813 2 View

What is the truth of Bcl-2 phosphorylation and its anti-apoptotic activity ?

Dose BcL-2 phosphorylation activate or inhibit its antiapoptotic function?. Is it still controversial?, because in some papers they says Phosphorylation at one or multiple sites within the...

31 May 2016 550 6 View

What is the best reagent to be applied on cultured primary VSMCs to mimic CaCl2 induced abdominal aortic aneurysm?

14 April 2016 9,577 0 View

What is the best way to inhibit beta catenin in vivo?

Hi, I need to inhibit beta-catenin in mice. Are there any inhibitors can be used in vivo to inhibit beta-catenin activity?

21 January 2016 325 2 View

How can I differentiate between Vascular smooth muscle cells (VSMC) and myofibroblasts in in vitro culture systems?

How to differentiate between Vascular smooth muscle cells (VSMC) and myofibroblasts in in vitro culture systems ? which marker is specific for VSMC ?

10 December 2015 3,397 4 View

How are PTEN and Akt pathway implicated in dilated cardiomyopathy? and What is really happening to cardiomyocytes during heart dilation?

Comparing with myofibroblast, it seems that cardiomyocyte has different pathways of prolifration and apoptosis. Dilated cardiomyopathy: Is that a result of degradation of extracellular matrix and...

10 April 2015 1,026 4 View