Which solvent do I need to use to resuspend taqman probes (FAM)? Water molecular grade is OK? same as any primer?
I usually use water, free-nuclease or molecular grade jeje is the same
Regards
We use water as well. Two more points. 5-10mM Tris ph7.5 also worked well (we did not try higher TRIS concentrations or higher pH values). Be carefull regarding the type of your tubes (you use to store the water in). Some plastic material contains fluorescent material (often in softeners) that may influence the probes. It becomes more or less important when you store your water over longer time periods in plastic-ware instead of in glass vials. Short term storage (in our hands) was no problem.
Nuclease free water is the best, because when you dissolve in buffer, if ph changes you primer get degrade.
We resuspend primers and probes in 1 X TE buffer. The TE buffer itself diluted in molecular grade water from 100x to 1X (Sigma)
@ Ram Nageena; The tris component is to sabalize Ph, thus preventing degradation.
I usually use molecular grade water to resuspend taqman probes and primers. it's best. pH changes may effect your primer and probes' binding.
Best Regard.
We usually make two solutions that work very well: a stock solution (100pM) diluted in 0,1 x
TE buffer and a working solution (5 or 10pM) diluted in nuclease free water.
All Best,
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