Hassan Hachim Naser

7 Questions 22 Answers 0 Followers

Questions related from Hassan Hachim Naser

How to optimize B2L expression recombinant protein?

I have get insolube protein at 1mMIPTG after 4 hours induction time. I need to find insolube protein for purification in Nikal column..my protein 21kDa.

10 October 2018 5,247 2 View

How to optimize B2L expression recombinant protein?

I have get insolube protein at 1mMIPTG after 4 hours induction time. How can I find solube protein for purification by his tag nikal colum.

10 October 2018 8,440 6 View

Hello every body, anyone know the steps for check my primers specifity (F: 5′-caatgggtgtgtttgctgtc-3′ and R: 5′-agccggtgctgtagagaaaa -3′) with PCR pro...

Hello every body, anyone know the steps for check my primers specifity (F: 5′-caatgggtgtgtttgctgtc-3′ and R: 5′-agccggtgctgtagagaaaa -3′) with PCR product size 233bp for housekeeping gene (glpF)...

11 November 2011 1,332 3 View

Best PCR thermocycler conditions

Hi every body, i have to tried to design a groups of primers to my work about detection of some genes in bacterium (brucella spp.) i used primer3 oline software to design of my... Show moreHi...

07 July 2011 9,419 4 View

PCR bands

Hello every body. i have problem in my work on PCR product , when i done PCR by using primers for one gene has 460bp product, i get multiple bands more than 4 bands. but i know use the correct pcr...

07 July 2011 9,332 50 View

PCR bands

Hello every body. i have problem in my work on PCR product , when i done PCR by using primers for one gene has 460bp product, i get multiple bands more than 4 bands. but i know use the correct pcr...

07 July 2011 9,702 3 View

PCR bands

Hello every body. i have problem in my work on PCR product , when i done PCR by using primers for one gene has 460bp product, i get multiple bands more than 4 bands. but i know use the correct pcr...

07 July 2011 4,087 20 View