Dear colleagues, my lab is considering to acquire the autoflex speed TOF/TOF. Our main application is proteomics but we are also interested in the Biotyper microorganism identification. Could you share your experience with this instrument?
Thank you very much for your answer Dr. Hopf.
About proteomics I was referring to protein sequencing in deed. We are interested in characterisation of novel peptides and proteins from different sources so the accuracy of the instrument is crucial. However, the microorganism identification is another application we are interested in. The university is currently investing in a G2 XS qTOF MS from waters but there are possibilities to get another instrument and we are analysing the autoflex as a possible choice. Thanks again for your reply.
Agreed, the AutoFlex speed is indeed an excellent instrument. However, for microorganism identification it's like fighting small things with heavy armory. As already mentioned, the performance of an AutoFlex speed equipped with the latest smartbeam technology (Nd:YAG laser) is not really required and btw. also not optimal for microbial identification. A MicroFlex instrument from Bruker with a N2 laser could also do the job. At the other hand, if your main application is proteomics I would recommend buying LC-MS/MS equipment (nLC-Orbitrap?).
Like always, the decision depends on the available budget. The two applications have different requirements and ideally one should consider buying two different instruments. This is what we did in our laboratory.
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