How do I interpret p and q in the corARMA correlation structure?

I understand that p is the autoregessive order and q is the moving average, but I don't know how to interpret what does it means when it is (2,3) or (2,2) for example. Can anyone give me a simple...

09 October 2018 6,260 0 View

Do you have any recomendations for a MALDI-TOF MS instrument?

I am exploring the possibility of buying of a Maldi-Tof MS instrument for my lab. I am familiar only with Bruker Maldi-ToF MS. My main application is characterization of novel peptides so accuracy...

02 March 2018 1,740 6 View

More sequencing is needed to profile functional diversity than phylogenetic diversity, is this true?

There is a commentary in Langille et al., 2013: Predictive functional profiling of microbial communities using 16S r RNA marker gene sequences. "More sequencing is needed to profile functional...

27 March 2017 6,996 3 View

Are tandem repeats of rDNA target for SSR markers?

15 February 2017 2,539 1 View

Is C value paradox applied only for genome size, or to genome complexity, as well?

C value paradox describes the lack of relationship between genome size and organismal complexity (Buchanan Biochemistry and Molecular Biology of Plants, 2nd Edition p. 405). I wonder if this...

08 February 2017 9,825 4 View

Could you recommend a company for custom peptide synthesis?

06 July 2016 4,556 3 View

How to differentiate if it is a new type of protein or a new variant of the same type?

I am wondering what is the % or number of Aa different to consider a protein as a new type or new variant?. I am working with new proteins (Kazal-type protease inhibitors). If you can suggest a...

03 March 2016 7,361 3 View

How to differentiate if it is a new type of protein or a new variant of the same type?

I am wondering what is the % or number of Aa different to consider a protein as a new type or new variant?. I am working with new proteins (Kazal-type protease inhibitors). If you can suggest a...

02 March 2016 3,904 4 View

Do you have any recommendations of a sofware or tool to align peptide fragments obtained by MS/MS?

25 September 2014 3,151 3 View

Do you have any recommendations of a sofware or tool to align peptide fragments obtained by MS/MS?

08 September 2014 1,408 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View