Ram Nageena Singh

4 Questions 21 Answers 0 Followers

Questions related from Ram Nageena Singh

Denovo chimera removal is recomended or not?

during the analysis of V3 region of 16S Next gene consensus. should I remove denovo chimera or proceed for clustering (OTU formation) without chimera? what should be the minimum % of identity (90,...

01 January 2015 3,061 2 View

Does anybody have methods for DNA-DNA hybridization for non-radioactive identification of microbes?

I want to do DNA-DNA (GDNA) hybridization for identification of bacteria. I wish to use non-radioactive method because my lab is not equipped for radioactive practices.

12 December 2014 7,462 3 View

Is there any pigment which gives spectra maxima at 245nm?

I am working on pigment extraction (carotenoid extraction) from Archaea and found a pigment which gives UV-visible spectra maxima at 245nm. It is a pink color pigment. Do you know what pigment...

12 December 2014 9,010 3 View

Mega plasmid extraction from bacteria?

Hello, If any body have verified protocol for extraction of Mega Plasmids from Bacteria, please share. I have tried to extract the Mega Plasmid from Gram negative bacteria but could not able to...

10 October 2014 2,038 2 View