Which software or a widely used bioinformatics tool can I use for gene structure?

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What is the reason that the binding energy of a docked complex is negative?

I have two questions: The binding energy of complex is negative. Why? can u suggest any tools to determine the interacting energies for a docked complex?

06 July 2018 10,132 0 View

What is the best absorbance value of Agrobacterium culture to be used to infect plant callus?

I need to know the best absorbance value that correlates the growth of Agrobacterium that can be used to infect plant callus. I have tried with 1.0 at 600 nm infection which helped me to transform...

01 February 2018 959 1 View

I carried out patchdoc for Protein-DNA and got the result but i need a tool for find interaction energy

I need to find MoleculeInteraction energyVander Waals energyElectrostatic energyHydrogen bond energySolvation free energyInteracting energy I have already tried autodoc since the ligand is a...

06 July 2017 4,693 2 View

Why do I get contaminations after two-three weeks sucessful plant callus growth?

I get contamination either fungal or bacterial after a two week successful establishment of Plant callus. Please tell me why it happens? Endophytes are ruled out totally since even after the...

31 December 2015 5,509 11 View

Why is E.coli BL21(DE3) strain used in eukaryotic expression?

why is it that only E.coli BL21(DE3) strain is widely used for eukaryotic gene expression when other strains can also be used. what is the full form of BL21(DE3). I searched but i did not find...

08 September 2015 4,472 4 View

How can I use the software "Map Inspect" to find locations of genes?

I want to know how to use map Inspect software. I tried searching publications related to Map Inspect protocol. but I was unable to find anything about the same. I need to know the...

04 May 2015 9,766 1 View

How can one estimate Salicylic acid and Jasmonic acid in plants using calorimetry?

How to determine calorimetrically? HPLC methods exist but can one determine using UV spec or fluorometry?

09 October 2014 2,027 3 View

What is the mechanism of WRKY transcription factor against biotic stress?

What is the probable mechanism of WRKY Transcription factor or the pathway in which wrky plays a major role against biotic stress. Please provide references.

08 September 2014 6,317 7 View

What are the new advanced techniques in plant molecular biology involved in proving disease resistance?

Need to know new methods which can help in proving the role of a particular gene in a specific function of the gene.

05 June 2014 3,673 2 View

Can fixing the gel be a problem for no spots in your 2D gel?

I get no spots in my 2D gel. Can not fixing it be an answer? I have tried everything but I cannot seem to fix this problem. Please advise.

04 May 2014 2,864 10 View

GC-MS retention index prediticon?

Hello experts, Does anyone know any free software about retention index prediction ?

08 August 2024 7,403 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

Can anyone provide me with molecular docking softwares/ websites?

Molecular docking software/ websites?

02 August 2024 8,704 7 View

Seeking Software Recommendations for SELEX NGS Data Analysis?

I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed

30 July 2024 1,061 5 View

CAD File of human's & rat's respiratory airways ?

Dear all, I am working on particle deposition in human's & rat's respiratory airways using CFD and I am looking for the 3D CAD file for my simulations (STEP or IGES format). If somone has such...

29 July 2024 1,092 2 View

I am working on my Master's thesis on the biogeography of the genus Ruagea and I would like to ask, could someone help me to check whether my result?

I created a file with my outgroup and ingroup species using Beauti, ran it in BEAST, viewed it in Tracer, and then used TreeAnnotator to create a file that I imported into RASP. Could someone...

28 July 2024 2,979 1 View

Could you try using PeptiCloud and see if it's a useful tool for biology research?

PeptiCloud (www.pepticloud.com) is a bioinformatic platform that allows researchers to organize and share their data for their projects as well as collaborate with others in one place. Through...

28 July 2024 4,762 2 View

Do you know of any online international conferences that offer free discussions?

Do you know of any online international conferences that offer free discussions? I am looking for examples in the field of molecular ecology and DIY biology.

28 July 2024 6,501 0 View