i am working on carotenoids and i want to calculate limit of detection, limit of quantification, accuracy, precision & method validation for beta-carotene. pls guide me for above.
You should not require any special software other that what is normally used to acquire and perform the data analysis (which you should have).
Most modern chromatography software packages include detailed reporting features which can calculate S/N ratios for baseline and reference peaks.
What's the detector?
You can refer to the following guidance for LC-MS.
https://www.fda.gov/downloads/drugs/guidances/ucm368107.pdf
you can use empower software or validated excel sheet, also don't hesitate to contact me to get excel sheet
Khanjan,
the abovementioned suggestions are good. Your software for HPLC should include a suitability test application - on this basis you can determine a lot of parameters used for validation, for example the S/N ratio for your analytes or resolution, retention factor etc.. At the end when you will set all parameters/criteria for your method this application can be used to control its fullfilment by defining some of your samples as the one for suitablity check in each series/batch of samples.
regards,
G
You should not require any special software other that what is normally used to acquire and perform the data analysis (which you should have).
Most modern chromatography software packages include detailed reporting features which can calculate S/N ratios for baseline and reference peaks.
Thank you @ William Letter & Grzegorz Boczkaj .
i am accessing empower hplc software
kindly suggest me that data analysis package or tool, which help me to calculate limit of detection & quantification, coefficient variance?.
Hi Khanjan,
Would you please tell, which instrument you are using currently? Actually, there are many software packages depending on different instrument. These softwares calculate the S/N ratios and according to the FDA guidelines for bioanalytical method validation the S/N ratio for LOD and LOQ is 3 and 5, respectively. I hope, Inside the software you would able to find everything.
Best wishes..
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