Maria Sokol

7 Questions 10 Answers 0 Followers

Questions related from Maria Sokol

How much tissue sample should I homogenize to study drug biodistribution?

I am working towards biodistribution study the first time now. And it is unclear for me how much tissue sample (lungs, liver, kidneys, heart, spleen, testicles) should I treat to homogenize and...

23 August 2018 647 2 View

Can anyone advise some literature on in vitro-in vivo release correlation?

Is it important to determine in vitro-in vivo correlation, when talking about PLGA nanoparticles release? Why it is important? Are there special criteria for nanoparticles suitablity in case of in...

29 July 2018 6,416 4 View

Whether the study of biodistribution is included in the pharmacokinetics?

I need to carry out pharmacokinetics study on rats by HPLC. Should I take drug from tissue samples for this purpose or only blood samples investigation will be enough?

15 June 2018 6,890 9 View

How can I increase coupling and yield of PLGA-COOH and peptide with two reaction sites?

I need to conjugate PLGA with carboxylic end and peptide (1061 Da). The peptide has two unprotected NH2-groups. When using EDC and NHS (PLGA:EDC:NHS = 1:5:5, molar ratio) the percentage of...

06 February 2018 2,450 3 View

How to enchence water solubility of docetaxel?

What system is it prefereble to use for determination of docetaxel LD50? I utilized dissolution system that is used for clinical application, but it is not sutible in my case because of little...

26 June 2017 1,564 5 View

How can I remove Boc protective group in prescense of Trt and TBS?

There are several protective groups in my peptide sequence: Fmoc, Boc, Trt, TBS, Dde, Cbz. How can I selectively remove Boc?

22 June 2017 8,223 2 View

Conditions for separating of basic and acidic compounds (HPLC)

I need to separate two compounds with pKa 5,19 and 10,96. Which buffer should I use and which pH is more suitable?

02 March 2017 5,865 3 View