Which simple sequence repeats (SSR) primers -with high polymorphic power - can I use to analysing date palm caltivars?

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Why we got more than one repeat for each primer used in NGS applied for DNA sequencing from each sample of date palm tree ?

Our project objective is to investigate the genetic diversity of Palestinian date palm, cultivars. 6 primers were ordered, these primers were designed specifically for NGS analysis. DNA was...

01 February 2020 2,887 1 View

Call for partners: Are there a scientific research partners interested in forensic biology?

In order to preparing a project proposal for Horizons ICT-33-2019. We have the first and only department in Forensic science at Al- Istiqlal University, Jericho-Palestine, also we are...

03 April 2019 9,499 6 View

I am looking for the best primers to use for genetic Identification of the date palm ?

Dear colleagues, I have a project: Genotypic, phenotypic and biochemical characterization of local date palm cultivars to identify the most economically and environmentally feasible cultivars....

11 December 2018 4,216 2 View

What is the best time in the year to extract DNA from date palm leaves?

I have a project with title: (Genotypic, characterization of local date palm cultivars to identify the most economically and environmentally feasible cultivars in Jericho- Palestine). I want to...

09 October 2018 9,875 1 View

I didn’t really understand how they got hepatocytes and other cell types from a live liver to a frozen vial?

I knew that we purchased primary hepatocytes and other cell types from vendors and that these cells are costly and precious, but didn’t really understand how they got from a live liver to a frozen...

03 April 2017 5,443 2 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Are there any instruments for studying time similar to the way it is in space?

There are a huge number of methods for studying objects in space, according to the senses (and not only). Mechanical, thermal, optical, acoustic, electrical, magnetic, based on particle beams,...

06 August 2024 7,102 0 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

How do microbial communities contribute to ecological cycles on Earth and how does an agroecological system support diverse microbial communities?

25 July 2024 5,197 2 View

Madhav Nepal

Walid Khalilia , here is good article on micro satellite markers for your plant Chapter Date Palm Genetic Diversity Analysis Using Microsatellite Polymorphism

Vijay Gahlaut

You can use following SSRs Article Development, characterization and use of genomic SSR markers...

https://www.frontiersin.org/articles/10.3389/fpls.2017.01889/full

Walid Khalilia

Thanks for your help Vijay Gahlaut

Thanks a lot for your help Madhav Nepal