The best culture condition for Kit-225

05 June 2017 6,445 6 View

How can I overcome such problematic melt curve in qPCR?

31 December 2015 5,690 5 View

How to achieve better amplification in qPCR?

09 October 2015 6,293 9 View

How might I ensure RT-PCR consistency ?

06 July 2015 6,551 5 View

Why is my western blot black and without bands?

I am currently doing western blot for p-Akt. Below is my protocol: -- Blocking = RT, 2 hours with 5% BSA -- Primary antibody = p-Akt (1:2000), overnight, RT -- Secondary antibody = Anti rabbit...

04 May 2015 6,166 9 View

What is the difference between non-fat milk and BSA for primary antibody in western blot?

Some protocols mention primary antibodies should be diluted in 5%BSA whereas some protocols mention primary antibodies should be diluted in 5% non-fat milk. What is the difference? What is the...

04 May 2014 387 20 View

Is serum starve important for TGF-beta induced EMT and EGF induced EMT?

Many publications mention that cells were serum starved for 24 hours before adding TGF-beta or EGF to induce EMT. After serum starved, is it possible to treat cells with EMT-inducers in RPMI 5%...

04 May 2014 7,714 10 View

Do you know where I can find cancer genomic data on kyse50, kyse 110, TE2, and TE3?

I have found that cbioportal is a good website to study molecular expression level of certain proteins in certain cancer cell lines. Unfortunately, some cell lines are not in the database. Where...

03 April 2014 1,016 4 View

Can anyone help: my western blot result of a snail protein is higher than 30 KD?

The supplier COA stated that the snail protein is about 30KD. I ran the electrophoresis at 200V, 125mA for 45mins. I used 4-12% Tris Nu-PAge Gel. Why is the snail protein more than 30KD?

02 March 2014 8,085 7 View

How can I improve my western blot results for the optimal dilution for a Snail-1 antibody?

I am currently comparing snail-1 expression in esophageal cancer cell lines. My protein load was 30-40 ug, the primary snail-1 antibody (cell signaling) was 1:1000, and the secondary anti-rabbit...

02 March 2014 8,669 17 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

How to accurately quantify chitin?

Hi all, I have been working on the quantification of chitin in insect samples and feed for a while but I can notice that the quantification methods (e.g. exposure to HCl, NaOH, and weighing sample...

01 March 2021 1,843 2 View