The best culture condition for Kit-225

05 June 2017 6,445 6 View

How can I overcome such problematic melt curve in qPCR?

31 December 2015 5,690 5 View

How to achieve better amplification in qPCR?

09 October 2015 6,293 9 View

How might I ensure RT-PCR consistency ?

06 July 2015 6,551 5 View

Why is my western blot black and without bands?

I am currently doing western blot for p-Akt. Below is my protocol: -- Blocking = RT, 2 hours with 5% BSA -- Primary antibody = p-Akt (1:2000), overnight, RT -- Secondary antibody = Anti rabbit...

04 May 2015 6,166 9 View

What is the difference between non-fat milk and BSA for primary antibody in western blot?

Some protocols mention primary antibodies should be diluted in 5%BSA whereas some protocols mention primary antibodies should be diluted in 5% non-fat milk. What is the difference? What is the...

04 May 2014 387 20 View

Is serum starve important for TGF-beta induced EMT and EGF induced EMT?

Many publications mention that cells were serum starved for 24 hours before adding TGF-beta or EGF to induce EMT. After serum starved, is it possible to treat cells with EMT-inducers in RPMI 5%...

04 May 2014 7,714 10 View

Can anyone help: my western blot result of a snail protein is higher than 30 KD?

The supplier COA stated that the snail protein is about 30KD. I ran the electrophoresis at 200V, 125mA for 45mins. I used 4-12% Tris Nu-PAge Gel. Why is the snail protein more than 30KD?

02 March 2014 8,085 7 View

How can I improve my western blot results for the optimal dilution for a Snail-1 antibody?

I am currently comparing snail-1 expression in esophageal cancer cell lines. My protein load was 30-40 ug, the primary snail-1 antibody (cell signaling) was 1:1000, and the secondary anti-rabbit...

02 March 2014 8,669 17 View

Which scale is better for the Measurement of Fluorescent Intensity?

Which scale is better, Linear scale or log scale?

02 March 2014 7,258 7 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View