Scaffold protein
Dear Murshidul:
Micelles are also a good option to make protein complexes to be further analysed.
They can be made with detergents or using different fatty acid mixtures.
Good luck
Rosa María Martínez-Espinosa
Hi, I want to use attached material in my HFSS simulation. In the spec sheet the DK was provided but instead of DF they provided FxQ. What value should I put in HFSS for loss tangent or DF? Can...
17 January 2024 1,749 1 View
I checked their author guidelines and I couldn't find a Proper answer
15 November 2023 5,644 2 View
Can someone please explain why low dielectric loss tangent (TanD) substrate cause narrower bandwidth for antenna and vice versa?
13 September 2023 1,359 1 View
Hi, I simulated an antenna with a matching network. Now to calculate the antenna range using Friis equation I need to know the losses due to the matching network. Can anybody suggest how can I...
18 May 2023 1,445 11 View
21 items 👉🏼 4 sub-constructs 👉🏼 1 construct 33 items 👉🏼 4 sub-constructs 👉🏼 1 construct so there are three levels: lower order 👉🏼 medium order 👉🏼 higher order The results are significant. But...
12 April 2023 5,185 8 View
I want to inquire about various methods to prove the contingency perspective in social sciences.
12 April 2023 3,328 1 View
Hi, I am designing UWB antenna. I know group delay variation is an important parameter. Is there any limit for GD variation. If yes, what is the acceptable group delay variation value for UWB...
29 November 2022 5,768 4 View
Hello Folks I was wondering if you kindly suggest me some free online tools to prepare graphs for academic/scientific documents. Thanks in advance for your cooperation.
28 October 2022 7,594 7 View
Please guide me how to remove this error.
27 October 2022 3,431 0 View
Few thing I need to know from everyone seeing this post for my research purpose. Please answer the following questions in the comment box. *People residing in Bangladesh only* . 1. Do you have AC...
05 September 2022 6,030 0 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC
01 August 2024 967 3 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...
30 July 2024 6,420 1 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...
27 July 2024 6,605 0 View