Which sample is used for standard immunofluorescent anti-nuclear antibody test ?

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Problem with my Proxy model.?

Hello everyone and thank you for reading my question. I have a data set that have around 2000 data point. It have 5 inputs (4 wells rate and the 5th is the time) and 2 ouputs ( oil cumulative and...

04 March 2024 2,248 2 View

As a marketing specialist, how do you see the future of digital marketing in Iraq in particular and the Arab world in general?

As a marketing specialist, how do you see the future of digital marketing in Iraq in particular and the Arab world in general? In light of the development of technology and the use of artificial...

05 January 2024 6,254 2 View

Error in CMG Builder when importing PVT model?

Hello everyone, and thank you in advance for reading/answering my question. I have built my gelogical model in PETREL software exported it into Rescue format and imported it into CMG Builder, to...

19 November 2023 3,672 1 View

Are azo compounds (mono) exist as two spots in TLC?

"When employing TLC to assess the purity of synthesized azo compounds, the presence of two spots prompts the question of whether these two spots are the result of geometrical isomers."

04 November 2023 724 0 View

I am trying to use PYTHON to run ECLIPSE reservoir simulator to find the optimum locations of oil wells in a field?

We have the ECLIPSE executable that we can call, there is an ECLIPSE input text file called input.DATA which includes well location in x and y directions. optimizer should change the x and y...

24 June 2023 5,972 1 View

Eclipse Simulator file structure?

Hello everyone and thank you for reading/answering my question. Is there a book, manual, video that talks about Eclipse file's structure and how to build my own file and run it in Eclipse. I...

21 June 2023 1,555 0 View

Can we use Petrel with Python?

Hello everyone and thank you for reading/answering my question. I have found that Petrel software can be used in conjunction with MATLAB to create plug in or access datasets and other features...

15 June 2023 9,741 5 View

Where are Petrel project Data stored in?

Hello everyone and thank you for reading/answering the question. 1) When we import our (PVT, Wells, Petrophysics...etc.) data into Petrel at which file does Petrel save them at? 2) Can I open the...

07 June 2023 850 3 View

Is there a Mathematical expression that relate Gamma ray to Resistivity?

Hello everyone I'm researching something for my master thesis and i needed an mathmatical expression or an equation that relate the gamma ray to Resistivity. Does such a equation exists in...

19 March 2023 1,772 3 View

Master thesis ideas for Petroleum Engineering?

Hello everyone I'm a master student and I'm about to start writing my thesis in Petroleum Engineering in a few months. So I have trouble in choosing my subject and what is trending right now in...

07 January 2023 2,394 4 View

Can I use a HisTRAP column for affinity chromatography?

I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...

07 August 2024 505 3 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to correctly use the UTE and ZTE pulse sequences in Bruker's ParaVision software?

I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...

01 August 2024 9,227 1 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

What is the reason I get 400nm nanoparticles using an extruder with 200 nm membrane for cell membrane coating?

I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...

30 July 2024 3,294 3 View

IHC profiler in image j software. has anyone used it to quantify nuclear DAB positivity?

Dear researchers. I tried using the IHC PROFILER in image j to quantify nuclear DAB staining. I followed the instructions in the original article by "Varghese F, Bukhari AB, Malhotra R, De A...

29 July 2024 2,229 0 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

While using the IHC PROFILER plugin in image j software, to quantify the epithelial cytoplasmic DAB staining do you crop remove the stromal tissue?

My question pertaining to the DAB staining in cytoplasm of human oral squamous cell carcinoma tissue. When quantifying the epithelial cancer cells do we have to crop remove the stromal tissue?...

29 July 2024 2,682 6 View

Problems with ion exchange chromatography?

I'm having problems with ion exchange chromatography. After applying my sample (a fluorescent protein), a preferential path inevitably forms. The matrix I use is DEAE-Sephacel. Could someone...

27 July 2024 6,605 0 View