What are the different way to give psychological (mental) stress in human population studies ?

01 February 2015 1,586 4 View

How to measure total body reaction time in human ?

I wan to measure total body reaction time in human

01 February 2015 394 0 View

Is there any possibility to differ auditory and visual reaction in different geographical region of same country ?

31 December 2014 7,464 3 View

Is it necessary to do GC-MS study for using crude extract to treat animals?

09 October 2014 2,834 8 View

What is the importance of ( df and F ) in one way ANNOVA analysis?

09 October 2014 2,971 4 View

What is the best method to isolate specific compound from plant extract?

09 October 2014 3,927 6 View

Can any one help me get a protocol for giemsa staining for bone marrow of wistar albino rats?

I want to do giemsa staining for bone marrow cells.

09 October 2014 8,393 5 View

Can anyone help me to get a protocol for cresyl fast violet staining for neuronal tissue?

I want to do cresyl fast violet staining for brain of wistar albino rats.

09 October 2014 550 1 View

What is the criteria for fixed dosing in vertebrates for any plant extract studies?

I want to know what the criteria should be to monitor the fixed dose for plant extracts on vertebrates (fishes).

08 September 2014 9,849 1 View

Can I use a zebra fish model to find out the brain antioxidant activity of aqueous extract of some fruit seed?

08 September 2014 8,225 3 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

No title

28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View