What are the different way to give psychological (mental) stress in human population studies ?

01 February 2015 1,586 4 View

Is there any possibility to differ auditory and visual reaction in different geographical region of same country ?

31 December 2014 7,464 3 View

Is it necessary to do GC-MS study for using crude extract to treat animals?

09 October 2014 2,834 8 View

What is the importance of ( df and F ) in one way ANNOVA analysis?

09 October 2014 2,971 4 View

What is the best method to isolate specific compound from plant extract?

09 October 2014 3,927 6 View

Can any one help me get a protocol for giemsa staining for bone marrow of wistar albino rats?

I want to do giemsa staining for bone marrow cells.

09 October 2014 8,393 5 View

Can anyone help me to get a protocol for cresyl fast violet staining for neuronal tissue?

I want to do cresyl fast violet staining for brain of wistar albino rats.

09 October 2014 550 1 View

What is the criteria for fixed dosing in vertebrates for any plant extract studies?

I want to know what the criteria should be to monitor the fixed dose for plant extracts on vertebrates (fishes).

08 September 2014 9,849 1 View

Can I use a zebra fish model to find out the brain antioxidant activity of aqueous extract of some fruit seed?

08 September 2014 8,225 3 View

Is it any one can help me to get protocol for spectophometer (manual) method for estimation of aminoacid phenyalanine and tyrosine.

08 September 2014 5,830 0 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

How to Eliminate cosine error and abbe error in LASER measurement system(for example Rhenishaw XL80)?

I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...

28 February 2021 4,890 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View