Which reagent can be recommended instead of disulfaphenol to determine the amount of nitrate in the soil?

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

HPLC baseline failure (ACN/Water)?

My baseline increases without any explanation, it is only fixed by changing the solution B, will it be the column? Reagents? Bomb? Detector? Solution A: H3PO4 0.01% IN WATER Solution B: H3PO4...

21 February 2021 5,656 3 View

What reaction will occur when adding hexamine into ammonium nitrate in an acidic medium?

Hello, I am investigating properties of water in oil emulsion explosives. And adding hexamine (hexamethylenetetramine) into one of the phases that are used to make the emulsion should increase the...

21 February 2021 8,680 1 View

Which transfection reagent is the best for plasmid transfection of MDA-MB-231 cell line, Lipofectamine based or other reagent suggestion?

I try to transfect MDA MB 231 cell line with a plasmid by PEI, unfortunately, the rate of transfection is low, and now I wanna find and order the best transfection reagent for this cell line and...

19 February 2021 828 6 View

Any information about dinitrosalisylic acid reagent end point color stability ?

I work on estimation of alpha amylase activity using dinitrosalisylic acid reagent If i put the reagent to stope the enzymatic reaction and allow the reagent to react with the produced reducing...

17 February 2021 7,402 4 View

Has anyone performed a colorimetric RT-LAMP assay using Lucigens LavaLAMP kit?

Hi there, my lab recently opted to use LavaLAMP from Lucigen instead of the Warmstart kit from NEB. However, Lucigen has yet to create a colormetric kit for RT-LAMP, and adding phenol-Red (pH...

17 February 2021 5,399 1 View

Are there alternatives to the Devarda alloy when estimating nitrogen?

Are there alternatives to the Devarda alloy when estimating nitrogen? devarda alloy is reducing the nitrate to ammonium

15 February 2021 8,838 3 View

How to inerpret whether PCR went OK?

Dear friends! I am back to work after 3 years career break. Now performing a PCR, feeling like a newbie... Please, help to understand whether RT-PCR reaction "worked" or not. Which results should...

14 February 2021 3,129 8 View

Abdelkader BOUAZIZ

Dear Otamurod Imomov, there are various alternative techniques with high accuracy. Please have a look at the following documents. My Regards

https://doi.org/10.2136/sssaj1981.03615995004500020024x

https://doi.org/10.1039/AN9679200311

https://link.springer.com/article/10.1007/s42452-020-3016-5

https://doi.org/10.1080/00103629009368334

Aref Wazwaz

Dear Otamurod Imomov . See the following useful links: https://dl.sciencesocieties.org/publications/sssaj/pdfs/45/2/SS0450020347

https://vlab.amrita.edu/?sub=2&brch=294&sim=1551&cnt=1

Kindly see also the following useful RG links: Article Determination of Available Nitrate, Phosphate and Sulfate in...

https://www.sciencedirect.com/topics/chemistry/griess-deamination

https://www.nrcs.usda.gov/Internet/FSE_DOCUMENTS/nrcs142p2_052828.pdf

Also check please the following useful RG link: Article The use of the Orange I method for determining soil nitrates...

The following useful RG link is also very useful: Article Humic Acid-Oxidizing, Nitrate-Reducing Bacteria in Agricultural Soils

Visit also the following useful RG links: Article Spectrophotometric Determination of Nitrate in Vegetables Using Phenol

https://dl.sciencesocieties.org/publications/sssaj/pdfs/45/2/SS0450020347