Hi all, I need to do ddCt analysis, I was trying to write a script for it, then found some R packages like HTqPCR or qPCR.CT . Do you have any suggestions for R packages? Which one is better?
I am not used to work in this research line but it is easy to find information about ddCt. For instance,
https://www.bioconductor.org/packages/3.3/bioc/vignettes/ddCt/inst/doc/rtPCR.pdf
as description over ddCt package in Bioconductor
https://www.bioconductor.org/packages/release/bioc/html/ddCt.html
In R you can look for ddCt and
https://cran.r-project.org/web/packages/qPCR.CT/qPCR.CT.pdf
Suggestions; as I said, I am not an "expert" in this techniques
For my research I used ReadqPCR and NormqPCR with great success.
Perkins J, Kohl M. (2011). ReadqPCR: Read qPCR data. Available at http://www.bioconductor.org/packages/ 2.14/bioc/html/ReadqPCR.html.
Kohl M, Perkins J. (2011). NormqPCR: Functions for normalisation of RT-qPCR data. Available at http://www.bioconductor.org/packages/devel/bioc/ html/NormqPCR.html.
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