30 November 2018 1 4K Report

I am planning the sorting of cancer stem cells from blood. There are more than 10 cell surface antigens. How can I choose the best ones to get the highest sensitivity and specificity?

More Ece Gumusoglu's questions See All
How can I apply FACS indirect staining protocol with biotin-tagged antibody and PE-streptavidin?

Hi everyone, I have a question regarding indirect FACS protocol. I'm using an unconjugated antibody which is biotin-tagged and PE-streptavidin as a secondary antibody. What should be their...

13 July 2023 4,603 3 View

Transwell assay with PBMCs?

It will be my first time doing this assay. I will be using PBMC's and as I gathered; some studies cover transwell surface for adhesion and I don't know if it is needed or how to do if needed....

23 May 2023 8,013 0 View

I got a notification of a pre-review version of my article in research gate with a doi number! Who can delete this from SSRN page and Reserachgate?

We had sent an article for review in May 2022 and it has been rejected. Recently I received a notification form Research gate asking me whether I am the author of the article which had a prereview...

07 February 2023 8,976 2 View

What are alternatives for cloning 2500 bp fragment into pET28a+ plasmid other than conventional cloning?

I am trying to clone 2461 bp fragment (restriction enzyme sites are included) into pET28a+ plasmid (which has his-tag that i will be using for purification of protein of interest later). So far,...

24 January 2023 6,612 7 View

Is it possible to see Estrogen Receptor Alpha and Beta upregulation via immunohistochemistry?

I am interested in a research idea that involves estrogen receptors. I need to see whether estrogen receptors are upregulated in the brain following my manipulation. However, most of the articles...

14 December 2022 1,341 1 View

Incudible system with stable transfection in mammalian cells?

Hello there, I would like to design a vector that will be incredible like TET-system but will be used to transfect the mammalian cells in the long-term. Would it be more beneficial to use shRNA...

31 May 2020 8,707 0 View

Do you have any idea or study about DC-DC boost converter control ?

I want to control the boost converter, found a transfer function but root locus of transfer func. shows that system is unstable so I cant use any controller. I attached the system's transfer func....

16 November 2018 4,037 7 View

Do you know the p.u conversion of DC motor in PSCAD?

I want to use DC motor and torsional shaft tools via PSCAD but rotational speed, mechanical torque and electrical torque have the unit of p.u. Do you know the base values ?

13 November 2018 2,700 4 View

Can you give me at least 5 kW DC motor parameters ?

I have desinged a dc boost converter to feed the dc motor and converter is powered by solar system but I have problem to determine the dc motor parameters. I use Pscad so there is no soft stater...

04 November 2018 4,345 0 View

What are the ways of measurement the stemness capacity of the cancer cells?

Hello, I would like to learn the measurement ways for the stemness capacity of the cultured cancer cell. I could not decide what is the best way to do this efficiently. Could anybody recommend...

01 November 2018 3,767 3 View

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Can I use a HisTRAP column for affinity chromatography?

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How to make a Lateral flow control line more uniform?

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31 July 2024 4,510 3 View

Can I proceed with Response Surface Methodology (RSM) with only one factor for further optimization?

I went for Plackett Burman Design for selecting the optimization factors of growth conditions for pesticide degradation. I started with 7 parameters. But, after the experiments and analysis, only...

29 July 2024 5,015 1 View

I did hirshfeld surface analysis for molecule but did not find red spot and interaction surface in energy frame work, is it ok for research article ?

In hirshfeld surface calculation i did not find any red spot in hirshfeld surface, is it ok for research article to write?

28 July 2024 2,988 0 View

Why specific capacitance is not proportional with the specific surface area?

Dear researchers, Why specific capacitance is not proportional to the specific surface area? I have material A with a surface area higher than material B, but material B is showing little bit...

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Why my IgG antibody looks fragmented on non-reducing SDS-PAGE?

I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...

23 July 2024 6,664 6 View

What are the strategies to Enhance IgG-Producing Plasma Cells in mice for Monoclonal Antibody Development?

I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...

22 July 2024 9,160 2 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

Differences between Surface Tension and Interfacial Tension in Liquid-Gas Phases?

Hi everyone, I have a question regarding surface and interfacial tension. Although I understand that these tensions are studied for different purposes, can they be considered equivalent in the...

18 July 2024 382 5 View

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