I am planning the sorting of cancer stem cells from blood. There are more than 10 cell surface antigens. How can I choose the best ones to get the highest sensitivity and specificity?
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I have desinged a dc boost converter to feed the dc motor and converter is powered by solar system but I have problem to determine the dc motor parameters. I use Pscad so there is no soft stater...
04 November 2018 4,185 0 View
Hello, I would like to learn the measurement ways for the stemness capacity of the cultured cancer cell. I could not decide what is the best way to do this efficiently. Could anybody recommend...
01 November 2018 3,677 3 View
I have been cloning TOPO vector containing 3'UTR of my gene of interest into pmirglo vector (7350 bp). I digested pmirglo and topo vector with XhoI and SacI and ligated them. I also digested...
14 May 2018 8,902 3 View
I cotransfected HEK293T cells with a plasmid containing 3'UTR of gene of interest and another plasmid containing miRNA sequence. I used equal amount for these two plasmids. After I did luciferase...
24 December 2017 5,936 1 View
Hi, I want to insert 130 bp DNA into 3000 bp vector, however I could not obtain colonies. I used 50 ng insert DNA and also tried to increase insert amount but still I could not observe any...
10 November 2016 3,279 5 View
I differentiated THP-1 cells with PMA into macrophages during 72 hours. Then, I washed with PBS and detached them from the flask. After I got the THP-1 derived macrophages, I cocultured them with...
10 July 2016 116 5 View
Ece Kaya is using my published book as hers and I cannot claim my own book in my profile. I would like to report this person and I kindly request removing my book from her profile.
01 January 1970 2,620 5 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...
02 March 2021 10,003 2 View
Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...
01 March 2021 3,867 3 View
We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...
01 March 2021 4,937 1 View
I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....
01 March 2021 3,607 3 View
Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?
28 February 2021 1,577 1 View
I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.
28 February 2021 6,512 3 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.
28 February 2021 5,929 2 View
I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...
25 February 2021 8,404 3 View