Removing duplicate SNPs in R?

Hello all, I am trying to perform SKAT to a dataset in RStudio. In order to do that, I first try to generate an SSD file; however I get the following error: "Check duplicated SNPs in each SNP...

17 September 2018 5,162 4 View

Gene deletion problem in cloning?

25 September 2016 5,787 2 View

What is the best method for extraction of plasmid DNA?

04 April 2016 7,963 10 View

What is the reason of weak band of plasmid on agarose gel electrophoresis?

I'm studying with pGFP vector for cloning. I have already cultured the plasmid and extracted DNA which is almost 300 ng/ ul. And now i'm trying to cut it with restriction enzyme. For the reaction...

30 March 2016 9,320 9 View

From where can we get temporal data of past 30 years of daily information of showing rivers channel flow?

I want to project for river change detection so may I know from where can I get these temporal data daily and weekly from the past 30 years and which data will be best suited.

18 January 2014 842 8 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

What are the best cations for preparing competent cells, Ca2+ or Mg2+?

I have been reading various articles and most state that treatment with CaCl2 produces higher efficiency of transformation than MgCl2. Can anyone explain why Ca2+ is better than Mg2+ at...

16 February 2021 8,519 5 View

How we can correct the slide?

i am doing research on E.feacalis that is grown in dentin and after that, we did a gram stain for the sample my Q first does the gram stain consider as reliable tool for testing and seeing the...

14 February 2021 6,697 1 View

Would using an asRNA for repressing transcription of a high expression Listeria monocytogenes non-coding RNA harbored in a plasmid be a viable option?

Hello all, I am interested in deleting a highly expressed non-coding RNA located on a Listeria monocytogenes plasmid. Currently, there are not any optimized gene deletion methods for Listeria...

09 February 2021 4,994 1 View

Review Papers regarding transcriptional regulations & promoter types/classes in Gram positive bacteria?

Dear fellow researchers, Could you please suggest me excellent review papers discussing transcriptional regulations and/or promoter classes in Gram positive bacteria? Thank you so much for your help!

08 February 2021 6,909 3 View

Flexibility of RNAseq?

When you use RNAseq I understand that it must follow certain conditions when you use it. One of them is the insertion of a polyA tail. The insertion of the polyA must include then...

05 February 2021 3,770 4 View

How do microbes tell the presence of an inorganic minaral in the soil?

Due to their ubiquitous nature, there is a tendency of the presence of microbes in a given mineral(s). For instance, if microorganisms that love the of pH less than 6 in the pH value, is isolated...

03 February 2021 4,630 10 View

Moving bacteria-like organisms in my HEK293T cell culture?

Hi everyone, I have been culturing HEK293T cells that have been engineered to overexpress the mechanosensitive protein Piezo1. However, I am finding it very difficult to keep them in culture -...

02 February 2021 7,892 4 View