How to make MRI image segmentation and create binary mask with imagej?

I have a number of MRI Dicom images. I need to create a binary mask with ROI for feature extraction. How I can do this by imagej?

25 December 2020 8,396 2 View

Is it possible to measure small temperature difference with K-type themocouple?

Hi, I am trying to measure temperature difference between inlet and outlet of a channel which is heated from upper wall. According to Q= m. Cp. DT formula 1 Celsius degree difference is expected...

29 May 2019 1,844 16 View

Removing duplicate SNPs in R?

Hello all, I am trying to perform SKAT to a dataset in RStudio. In order to do that, I first try to generate an SSD file; however I get the following error: "Check duplicated SNPs in each SNP...

17 September 2018 5,162 4 View

Which breast cell line makes baso-lateral membrane,tight junctions and has high polarity?

To know polarity defect (by knocking out some specific genes) I am looking for a breast cancer cell line that has high polarity, forms tight junctions and easy to transfect.

25 July 2018 9,592 3 View

How can I calculate friction factor in hydrodynamically developing flow?

As it is known if pressure drop between inlet and outlet of a geometry is known friction factor can be calculated from equation below; Dp = f (L\D) (ro U2\2) But use of this equation is only...

20 March 2017 909 3 View

Gene deletion problem in cloning?

25 September 2016 5,787 2 View

What is the best method for extraction of plasmid DNA?

04 April 2016 7,963 10 View

What is the reason of weak band of plasmid on agarose gel electrophoresis?

I'm studying with pGFP vector for cloning. I have already cultured the plasmid and extracted DNA which is almost 300 ng/ ul. And now i'm trying to cut it with restriction enzyme. For the reaction...

30 March 2016 9,320 9 View

How cationic liposome escape from endosome after been taken into the cell?

20 February 2016 203 1 View

Can someone explain more details why poly(N-isopropylacrylamide) (PNIPAAm) show LCST behaviour in water?

17 February 2016 4,943 4 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Has anyone tried to scan fluorescence IHC slides with microarray scanner?

I have an old slide microarray scanner (VIDAR Revolution 4200) and would like to give it a "second life", as IHC scanner array (fluorescence dyes), FISH over tissue or similar. Has anyone tried...

24 February 2021 9,210 1 View

Recommendations on homogenizers for RNA isolation?

I will be performing RNA isolation with frozen lung biopsy samples and am in the market for a tissue homogenizer. I am wanting a homogenizer that will fit in a 1.5ml tube. I was looking at the...

24 February 2021 3,623 3 View

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22 February 2021 9,932 11 View

How to locate and collect the pineal gland in the amphibian brain?

Our team has been trying to locate and collect the pineal gland in the American bullfrog's brain (Lithobates catesbeianus) for some time now. However, we don't have a standardized protocol for...

18 February 2021 3,453 4 View