Inci Basak Mustak

5 Questions 11 Answers 0 Followers

Questions related from Inci Basak Mustak

How to get rid of plasmid from the bacteria?

I study with a Salmonella strain. This strain has plasmid which carries amp resistance gene. And i want to get the strain free of plasmid. How to get it? any ideas?

20 March 2017 8,001 8 View

Gene deletion problem in cloning?

First, i PCR amplyfied my gene (~ 2500bp) and then cloned it to the bacteria (DH5@). Second, i digested it with restriction enzymes (blunt end, ~1300bp) and left flanking sites (~1200bp) inside...

26 September 2016 5,850 2 View

What is the best way to create blunt end?

I have a sticky- end plasmid and want to create blunt- end from it. Which one is good for reaction, to use DNA Polymerase I or T4 DNA Ligase?

26 May 2016 4,532 8 View

What is the best method for extraction of plasmid DNA?

I use commercial kits for extraction of plasmid DNA. But i don't have high plasmid DNA. For ex. i have tried MN (Macherey Nagel) NucleoSpin® Plasmid, Zyppy™ Plasmid (ZymoResearch) kits and got...

05 April 2016 8,044 10 View

What is the reason of weak band of plasmid on agarose gel electrophoresis?

I'm studying with pGFP vector for cloning. I have already cultured the plasmid and extracted DNA which is almost 300 ng/ ul. And now i'm trying to cut it with restriction enzyme. For the reaction...

31 March 2016 9,384 9 View