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Questions related from Inci Basak Mustak
Hi I'm trying to find a protocol for Staphylococcus aureus subsp. anaerobius PFGE. Do you know any PFGE protocol for Staphylococcus or any gram positive bacteria?
17 April 2018 6,266 8 View
I study with a Salmonella strain. This strain has plasmid which carries amp resistance gene. And i want to get the strain free of plasmid. How to get it? any ideas?
20 March 2017 8,007 8 View
First, i PCR amplyfied my gene (~ 2500bp) and then cloned it to the bacteria (DH5@). Second, i digested it with restriction enzymes (blunt end, ~1300bp) and left flanking sites (~1200bp) inside...
26 September 2016 5,858 2 View
I have a sticky- end plasmid and want to create blunt- end from it. Which one is good for reaction, to use DNA Polymerase I or T4 DNA Ligase?
26 May 2016 4,539 8 View
I use commercial kits for extraction of plasmid DNA. But i don't have high plasmid DNA. For ex. i have tried MN (Macherey Nagel) NucleoSpin® Plasmid, Zyppy™ Plasmid (ZymoResearch) kits and got...
05 April 2016 8,060 10 View
I'm studying with pGFP vector for cloning. I have already cultured the plasmid and extracted DNA which is almost 300 ng/ ul. And now i'm trying to cut it with restriction enzyme. For the reaction...
31 March 2016 9,395 9 View