I am trying to see the changes of the stability of predicted protein structures after certain amino acids substitution.
I am studying large-scale granite structures, specifically inselbergs. I would like to know your suggestions for analyzing these granite structures using remote sensing. Books, articles, and...
17 June 2024 6,687 1 View
Hello everyone, I'm currently working on a project where I need to isolate viruses in culture from incubation with PCR-positive serum samples, but I'm facing the challenge of dissociating...
17 June 2024 1,855 1 View
I want to sequence the genome of Chilean powdery mildew isolates from grape leaves using the Oxford Nanopore platform. Thanks in advance for your kind response.
17 April 2024 4,863 2 View
This question is based on research done by Kavulavu et al. et al. in 2022, entitled "Challenges facing blood transfusion services at a regional blood transfusion center in Western Kenya." In this...
10 April 2024 9,791 0 View
So I am trying to live-image the synapses of NK cells with either A549 or K562 cells. For that I am going to stain NK cells with lysotracker, cancer cells with membrane stain, and then add a dead...
19 March 2024 1,098 1 View
Hello. I'm part of a team that is conducting a qualitative study about the perspectives and opinions around social development. The participants of this phase were members of a rural community in...
27 February 2024 966 3 View
we have done a PCR for adhesion molecules comparing lung tissue from healthy mice to inflamed mice. surprisingly, the RNA expression in the inflamed mice is much lower than the healthy ones. I...
16 October 2023 9,447 1 View
Is divulge our duty? Are we responsible of keep population concerned about science and diversity?
16 August 2023 2,086 9 View
Publics institutions like goverments agencies or ministeries or local authorities recurrently can´t develop their planned projects on time and within schedule and cost. Could a project management...
07 August 2023 8,296 2 View
Im having troubles with Luna stain for eosinophils, the scarlet Beibrich is not working
04 July 2023 3,887 1 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...
05 August 2024 454 3 View
Dear readers, Thanks for your attention. I am wondering about the health economic problem of quantifying the value of interventions which a) prevent, b) improve symptom profile and c) ultimately...
05 August 2024 3,246 1 View
I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...
05 August 2024 6,787 4 View
I am working on a SEM model where i have 7 latent variables (6 exogenous and 1 endogenous). In AMOS when I co-vary the exogenous constructs, only 2 paths are coming significant out of 6. But when...
03 August 2024 6,028 4 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...
31 July 2024 2,033 1 View
Suppose I am studying the construct of "Academic Motivation" (latent variable) in students, which is measured through three observed variables: "Intrinsic Motivation," "Extrinsic Motivation," and...
31 July 2024 3,913 6 View