Hi, I´m quite new in the field of MALDI Imaging, could anybody give me a short feedback of experience of using one of these methods?
Tank you so much!
Hi,
I'm also not an expert in the field, but FT-ICR provides a higher mass resolution compared to TOF-MS (which could mean that it takes a bit longer to acquire a spectrum).
The spatial resolution however, which is determining what features can be distinguished in an image, is determined by the laser you use for desorption/ionization. So it's all about what you're looking for in an imaging experiment, I guess.
Hope this helps!
FTICR is generally higher resolution but slower to process each scan, meaning longer times to process a complete sample. Sensitivity can also be an issue. In general FTICR of higher mass species (>20,000) can also be very problematic because the isotopic pattern cancels out the signal for the FT to pick up. You don't have that limitation in TOF. The bottom line will be what is the mass range of the analytes you are expecting to see. If above 20,000 Da, then you must go TOF.
Hi Henriette, I totally agree with the previously mentioned suggestions I’m working with both
systems,
Depends a lot of what you are going to analyse and what is your major goal, if you are targeting small molecules < 1000 Da than I would totally go for the FT-ICR since you need the higher resolution, you could also combine the systems
Best regards
Bogdan
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