Henriette Uthe

5 Questions 3 Answers 0 Followers

Questions related from Henriette Uthe

WHich influence has benzonase on the unspecific binding character of proteins to magnetic beads?

We perform Co-Immunoprecipitation with HA-tag and anti-HA magentic beads (Pierce).We have a mild washing protocol (4 washing steps , 5min) and usally we detected many proteins binding unspecific...

28 April 2017 5,288 2 View

Which pros and cons has MALDI-FT-ICR MS compared to MALDI-ToF MS?

Hi, I´m quite new in the field of MALDI Imaging, could anybody give me a short feedback of experience of using one of these methods? Tank you so much!

12 April 2017 4,639 3 View

Differences in the sequence of maturating ribosomal proteins?

Hi everybody, does anybody know if there is a difference in the protein sequence or modification of ribosomal proteins which are in the nucleus (meaning during maturation and assambly with the...

06 March 2017 7,307 1 View

Is there a difference between aceton precipitation with Tris and Hepes buffer samples?

Hey, I performed several acetone precipitations with eluates (1ml) of my IP´s in 50 mM Tris buffer. I usally dissolve the pellets in 1xSDS buffer and load a SDS-gel. Now I had to switch to a...

08 April 2015 7,689 3 View

What is the best anti FLAG antibody?

Hi, i´m looking for a very specific anti-FLAG antibody to couple on magnetic beads. I tried the antibody from Sigma and Pierce, but they seems to be very unspecific in the western blot. Does...

14 January 2015 1,457 10 View