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Questions related from Henriette Uthe
We perform Co-Immunoprecipitation with HA-tag and anti-HA magentic beads (Pierce).We have a mild washing protocol (4 washing steps , 5min) and usally we detected many proteins binding unspecific...
28 April 2017 5,279 2 View
Hi, I´m quite new in the field of MALDI Imaging, could anybody give me a short feedback of experience of using one of these methods? Tank you so much!
12 April 2017 4,628 3 View
Hi everybody, does anybody know if there is a difference in the protein sequence or modification of ribosomal proteins which are in the nucleus (meaning during maturation and assambly with the...
06 March 2017 7,300 1 View
Hey, I performed several acetone precipitations with eluates (1ml) of my IP´s in 50 mM Tris buffer. I usally dissolve the pellets in 1xSDS buffer and load a SDS-gel. Now I had to switch to a...
08 April 2015 7,674 3 View
Hi, i´m looking for a very specific anti-FLAG antibody to couple on magnetic beads. I tried the antibody from Sigma and Pierce, but they seems to be very unspecific in the western blot. Does...
14 January 2015 1,446 10 View