I'm looking for the syngeneic cell line ENU1564 rat mammary adenocarcinoma cells. Does anyone have them, preferably in the USA, and are willing to share with me?
Thank you.
I was missing these cell lines (there are 3 of them) in the Cellosaurus, so I prepared entries for them and "digged" a bit on where you can get them. It looks to me that groups using them all have got them from the lab that created them, that of George Stoica at TAMU. So you will need to contact him and I guess you will need to indicate which one you need as they have different phenotypes. See below.
Best
Amos
ID ENU1564 FP1
AC CVCL_C8F4
RX PubMed=8039303;
CC Characteristics: Spontaneously metastasizes to regional lymph nodes and lung following orthotopic inoculation into mammary fat pad (mfp) and metastasizes widely following left cardiac ventricle (LV) inoculation (PubMed=8039303).
CC Doubling time: 12 hours (PubMed=8039303).
CC Transformant: ChEBI; CHEBI:23995; N-ethyl-N-nitrosourea (ENU).
CC Derived from sampling site: Mammary gland. Cell type=Epithelial cell of mammary gland.
CC Breed/subspecies: Berlin-Druckrey IV (BD-IV).
DI NCIt; C64011; Adenocarcinoma of the rat mammary gland
OX NCBI_TaxID=10116; ! Rattus norvegicus (Rat)
SX Female
AG 400D
CA Cancer cell line
//
ID ENU1564 Br7-C5
AC CVCL_C8F5
RX PubMed=7912494;
RX PubMed=8039303;
CC Characteristics: Highly metastatic. Obtained after seven passage of the parent cell line by inoculation in the left cardiac ventricle of rats and culturing of the resulting brain metastases (PubMed=7912494).
CC Doubling time: 12.8 hours (PubMed=8039303).
CC Transformant: ChEBI; CHEBI:23995; N-ethyl-N-nitrosourea (ENU).
CC Derived from sampling site: Mammary gland. Cell type=Epithelial cell of mammary gland.
CC Breed/subspecies: Berlin-Druckrey IV (BD-IV).
DI NCIt; C64011; Adenocarcinoma of the rat mammary gland
OX NCBI_TaxID=10116; ! Rattus norvegicus (Rat)
HI CVCL_C8F4 ! ENU1564 FP1
SX Female
AG 400D
CA Cancer cell line
//
ID ENU1564 FP2-A11
AC CVCL_C8F6
RX PubMed=7912494;
RX PubMed=8039303;
CC Characteristics: Highly metastatic. Obtained by reculturing the @parent cell line from a mammary fat pad (mfp) tumor. Grows slowly (PubMed=7912494).
CC Doubling time: 27 hours (PubMed=8039303).
CC Transformant: ChEBI; CHEBI:23995; N-ethyl-N-nitrosourea (ENU).
CC Derived from sampling site: Mammary gland. Cell type=Epithelial cell of mammary gland.
CC Breed/subspecies: Berlin-Druckrey IV (BD-IV).
DI NCIt; C64011; Adenocarcinoma of the rat mammary gland
OX NCBI_TaxID=10116; ! Rattus norvegicus (Rat)
HI CVCL_C8F4 ! ENU1564 FP1
SX Female
AG 400D
CA Cancer cell line
//
Thank you very much Amos Bairoch for the information. I did contact Professor Stoica, who is now retired, and he reached to colleagues at his former institution, but unfortunately, they said they don't have those cells anymore :(
Dear Henriette
Many thanks for the feedback. For the next release of the Cellosaurus I will indicate that these cell lines are no longer available.
Best
Amos
Hi Amos,
There is a lab at the University of Oxford (UK) that has the cells. But we have not yet been able to get a vial shipped to us. Still working on that, hopefully we will be able to obtain some.
Thank you.
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