Is it ok to flash freeze the cells for protein expression without losing protein?

HI, I am working to express proteins from pichia. Can I flash freeze the cells and store at -80 degree? Does protein get denatured after freeze and thaw? Do I need to proceed to protein extraction...

03 April 2018 6,601 6 View

How to remove detergent DDM from protein for protein crystallization?

Hi, Can you please tell me how to remove detergent DDM from protein sample for protein crystallization purpose? Thanks. 1, combine protein together and inject into SEC. 2, use lowest mwco filter...

02 March 2018 3,045 1 View

Help with sf9 cells culture and growth?

I took new ordered sf9 cells from the vendor and I did not following steps: 1, take the cells out from the vial, thaw, and add to a small stationary dish flask. The first day I can see majority of...

09 October 2017 178 3 View

How can I further purify my protein?

I am working on a membrane protein. After I mix the lysate from the solubilization of membrane from pichia yeast and nickel beads, I washed thoroughly with 20 mM and 40 mM imidazole, and elute...

09 October 2015 2,218 10 View

Who is familiar with working with proteoliposome?

Hi, I am a postdoc working with proteoliposome. However, I have not been able to make proteoliposome with my membrane protein. Here is my general procedure. a, dry the lipid as the thin film on...

08 September 2014 5,117 3 View

Problem of lysis of yeast cells

Hi, everyone, what is the best way to lysis yeast cells such as pichia pastoris or saccharomyces? i used bead beater and french press and found that they both cause heat to denature the...

01 January 1970 2,241 5 View

Does the pressure(-600 mmHg) affects pH?

When I conducted the degassing experiment using decompression under anaerobic condition(and I used ferrous based chelating agent), the oxidized ferric were observerd. So, could you tell me the...

03 March 2021 4,892 5 View

How the parameters (such as Mw, Chemical bonds, polymer bland and so on) affect the mechanic property of polymer (such as storage, loss modulus) ?

Anyone can recommend any books on such topics? Thanks in advance.

02 March 2021 8,457 2 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

Which database would give me this list of chemical components?

Dear colleagues: I would like to know if anyone knows of a database that gives me the parameters pH, K, K, Mg, P, C, N, Zn, Cu, Ni and Cd of common foods. I find a lot of information of proteins,...

26 February 2021 5,525 3 View

Why is NH4OH added in heavy metals limit test?

The limit test I'm referring to is USP method II. In this procedure you need to dropwise add 6N of NH4OH to the sample until it the pH is alkali. After that you need to dilute and add HOAc until...

23 February 2021 2,049 3 View

I am looking for a simple software program to model 3D chemical bonds. The purpose is to generate high-quality graphical abstracts. Any suggestion?

Hello everyone: I am looking for a simple software program to model chemical bonds. The purpose is to generate a high-quality graphical abstract for a top leading journal in the field of...

23 February 2021 8,012 3 View

How to use protein A G beads to purify antibody?

Secretory universal influenza antibody CR9114 were successfully expressed by adenovirus vector ,then we used protein A G beads purify CR9114.But the molecular weight of the purified antibody under...

22 February 2021 4,318 2 View

Is there an ideal incubation time of RGO-modified SPCE before subsequent use, i.e., electrochemical characterization or further modification?

I notice that upon washing and air-drying of my RGO/SPCE surface and characterizing again via DPV, the current response towards ferricyanide probe significantly decreases. My worry is that the...

22 February 2021 5,324 3 View

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22 February 2021 9,932 11 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View