Hi there,

any mechanical lysis tends to heat up the sample so you have to adapt the condition to avoid it. To improve the yield of lysis you could prepare spheroplasts in the first move.

You could try a freeze thaw cycle (or multiple cycles). I use this for rupturing cells for DNA extraction. Suspend cells in a buffer and freeze in liquid N or -80C for an hour then thaw at room temperature

add 3 mL 4 molar HCl solution and place in rotary water bath 79 degree c for 1 hour. then centrifuge

to rasool, i need to purify proteins so I can not use HCl in the lysis

oh okay, I was thinking you just want to break the cell as I do this for lipid extraction