Hai Li

7 Questions 5 Answers 0 Followers

Questions related from Hai Li

Is it ok to flash freeze the cells for protein expression without losing protein?

HI, I am working to express proteins from pichia. Can I flash freeze the cells and store at -80 degree? Does protein get denatured after freeze and thaw? Do I need to proceed to protein extraction...

04 April 2018 6,756 6 View

How to remove detergent DDM from protein for protein crystallization?

Hi, Can you please tell me how to remove detergent DDM from protein sample for protein crystallization purpose? Thanks. 1, combine protein together and inject into SEC. 2, use lowest mwco filter...

03 March 2018 3,107 1 View

Which pH does disulfide bond form preferably, high pH (~8) or low pH (~6)?

which pH does disulfide bond form preferably, high pH (~8) or low pH (~6)?, between the protomer of a dimeric protein? Thanks.

02 February 2018 6,666 1 View

Help with sf9 cells culture and growth?

I took new ordered sf9 cells from the vendor and I did not following steps: 1, take the cells out from the vial, thaw, and add to a small stationary dish flask. The first day I can see majority of...

10 October 2017 212 3 View

How can I further purify my protein?

I am working on a membrane protein. After I mix the lysate from the solubilization of membrane from pichia yeast and nickel beads, I washed thoroughly with 20 mM and 40 mM imidazole, and elute...

10 October 2015 2,253 10 View

Who is familiar with working with proteoliposome?

Hi, I am a postdoc working with proteoliposome. However, I have not been able to make proteoliposome with my membrane protein. Here is my general procedure. a, dry the lipid as the thin film on...

09 September 2014 5,154 3 View

Problem of lysis of yeast cells

Hi, everyone, what is the best way to lysis yeast cells such as pichia pastoris or saccharomyces? i used bead beater and french press and found that they both cause heat to denature the...

01 January 1970 2,275 5 View